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Fig. 3 | Alzheimer's Research & Therapy

Fig. 3

From: Neuroinflammation and amyloid-beta 40 are associated with reduced serotonin transporter (SERT) activity in a transgenic model of familial Alzheimer’s disease

Fig. 3

Inhibition of SERT activity by soluble Aβ40. a Forebrain membrane preparations from 3-month-old wild-type mice were incubated for 5 min with increasing concentrations of [3H]5-HT (20 nM-2.5 μM), in the presence or absence of human amyloid-β (Aβ)40/42. Peptide concentrations of 100 nM were used, based on the levels of soluble Aβ40/42 measured in the neocortex of 20-month-old APPswe/PS1dE9 mice by Meso Scale Discovery. Aβ40 reduced the apparent maximal velocity rate (Vmax) of [3H]5-HT uptake, without changing the apparent Michaelis constant (Km). Aβ42 had no effect on the uptake kinetics of [3H]5-HT. Results represent the mean ± SEM values of three independent experiments, each conducted in triplicate. b Forebrain membrane preparations were incubated for 2 h with 2 nM [3H]DASB, in the presence or absence of increasing concentrations of Aβ40/42 (0.01 pM-10 μM). The inhibitory effects of Aβ40 were not due to competition at the [3H]DASB-binding site

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