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Fig. 1 | Alzheimer's Research & Therapy

Fig. 1

From: A two-step immunoassay for the simultaneous assessment of Aβ38, Aβ40 and Aβ42 in human blood plasma supports the Aβ42/Aβ40 ratio as a promising biomarker candidate of Alzheimer’s disease

Fig. 1

Preconcentration of Aβ peptides from EDTA plasma facilitates semi-quantitative measurement of a Aβ42, b Aβ40 and c Aβ38 in human blood plasma. Bars indicate calculated concentrations in 4-fold diluted EDTA-plasma samples (black bars) and in 10-fold or 20-fold diluted IP eluates (light and dark gray bars) obtained by magnetic bead immunoprecipitation from 800 μl of plasma. Signals above LLOQ of the assay were observed after immunoprecipitation in five out of five tested samples for Aβ42 and Aβ40 and in four out of five samples for Aβ38. Indicated LLOQs were taken from lot-specific certificates of analysis provided with assay kits. Mean ± SD of duplicate reads shown. Aβ amyloid beta, IP immunoprecipitation, LLOQ lower limit of quantification

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Alzheimer's Research & Therapy

ISSN: 1758-9193