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Table 1 In-vitro binding characteristics of SAR2559952, humanized SAR228810, and comparators

From: SAR228810: an antibody for protofibrillar amyloid β peptide designed to reduce the risk of amyloid-related imaging abnormalities (ARIA)

Mouse IgG ELISA Aβ ELISA aggregated proteins
EC50 ng/ml EC50 ng/ml
Mean EC50 Protofibril (soluble) LMW/monomer Fibril/aggregates IAPP fibrils Synuclein fibrils
 SAR255952 36 ± 5.5 3751 ± 130 68 ± 15 NB up to 100 μg/ml NB up to 100 μg/ml
 3D6 61 ± 4.4 86 ± 6 45 ± 17 NB up to 100 μg/ml NB up to 100 μg/ml
Human IgG ELISA Aβ ELISA aggregated proteins SPR
EC50 ng/ml EC50 ng/ml Kd (M)
Protofibrils (soluble) LMW/monomer Aβ fibrils IAPP fibrils Synuclein fibrils Aβ monomer Aβ protofibrils (soluble)
 SAR228810 37 ± 2 14,403 ± 3403 16.7 ± 3.4 NB NB NB up to 1000 nM 4.3 ± 5 × 10–10
 3D6 human IgG 32 ± 2 NA 7.7 ± 2.2 NA NA 1.7 ± 1 × 10–8 < 10–10
 Gantenerumab 26 ± 8 NA 19.9 ± 4.7 NA NA 3.4 ± 3 × 10–5 8 ± 3 × 10–10
 BAN-2401 21.3 ± 9 NA 15.5 ± 5.1 NA NA 8.7 ± 4 × 10–6 < 10–10
  1. Binding experiments performed three to five times, mean ± SEM EC50 values are presented
  2. amyloid β, ELISA enzyme-linked immunosorbent assay, IAPP islet amyloid polypeptide, LMW low molecular weight, NA not available, NB no binding,