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Fig. 5 | Alzheimer's Research & Therapy

Fig. 5

From: Active full-length DNA Aβ42 immunization in 3xTg-AD mice reduces not only amyloid deposition but also tau pathology

Fig. 5

Quantitative enzyme-linked immunosorbent assay (ELISA) analyses for amyloid-β 1–42 peptide (Aβ42) and Aβ40 in brain lysates from triple-transgenic Alzheimer’s disease (3xTg-AD) mice. a Analyses of an increase of Aβ42 and Aβ40 peptides in brains from 3xTg-AD mice with age (12-month-, 18-month-, and 20-month-old female control mice). b Reduction of Aβ42 and Aβ40 peptide concentrations in the nonsoluble fractions of the brain lysates owing to Aβ42 immunotherapy. Blue bars show Aβ42 peptide concentrations found in brains from DNA Aβ42 trimer-immunized mice; yellow bars show the concentrations found in brains from Aβ42 peptide-immunized mice. The black bars show Aβ42 peptide concentrations in age- and gender-matched 3xTg-AD control mice. The left-hand graph displays data for Aβ42 peptides, and the right-hand graph shows data for Aβ40 peptides. c Reduction of Aβ42 and Aβ40 peptide concentrations in the soluble fractions of the brain lysates owing to Aβ42 immunotherapy. The left-hand graph shows data for Aβ42 peptides, and the right-hand graph displays data for Aβ40 peptides. ELISAs for the nonsoluble brain lysates were performed three times (dilution 1:10,000), and ELISAs for the detergent-soluble brain lysates were performed twice (dilution 1:2) for this particular group of mice and confirmed the data shown. * p  ≤0.05, ** p ≤ 0.01, *** p ≤ 0.005, and **** p  ≤ 0.001 (Mann-Whitney U test)

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