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Fig. 3 | Alzheimer's Research & Therapy

Fig. 3

From: Active full-length DNA Aβ42 immunization in 3xTg-AD mice reduces not only amyloid deposition but also tau pathology

Fig. 3

IHC staining of T231p (AT180) and T202p/S205p (AT8) in triple-transgenic Alzheimer’s disease (3xTg-AD) mouse brains and Western blots for total tau. a The age progression of T231p (AT180) in 3xTg-AD mouse brains is shown by IHC and staining in 2-, 4-, 7-, 9-, 12-, and 18-month-old mice. b T231p staining in the hippocampus of three brains from 18-month-old 3xTg-AD mice that had received DNA amyloid-β 1–42 peptide (Aβ42) trimer immunizations is shown for comparison. c Semiquantitative analyses for pT231 staining in the hippocampus of four 18-month-old control mice, five 18-month-old DNA Aβ42 immunized mice, six 18-month-old Aβ42 peptide-immunized mice, and four wild-type mice using ImageJ software (National Institutes of Health, Bethesda, MD, USA). Blue bars show positive areas found in DNA Aβ42 trimer-immunized mice, and yellow bars show areas found in brains of Aβ42 peptide-immunized mice. Black bars show the values of age- and gender-matched 3xTg-AD control mice. df DNA Aβ42 trimer immunization decreased AT8 staining in hippocampal sections from 20-month-old 3xTg-AD mice. d Representative sections from two control mice. e Sections from Aβ42 peptide-immunized mice. f Staining of AT8+ tangles in the hippocampus of two DNA Aβ42-immunized mice. All pictures are in 10× magnification (hippocampus); insets are in 40× magnification (subiculum). g A representative Western blot from detergent-soluble brain lysates of 20-month-old 3xTg-AD control mice (labeled C1–C4), DNA Aβ42-immunized mice (labeled D1–D4), Aβ42 peptide-immunized mice (labeled P1, P2), and a wild-type control (wt) mouse is shown. h Gray value intensities of human tau bands (indicated with an arrowhead, missing in the wt control, at 50 kD) were semiquantitatively analyzed using ImageJ software. Black bars show the values in 3xTg-AD control mice; yellow bars represent the peptide-immunized mice; and blue bars show values found in DNA Aβ42-immunized mice

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