Fig. 2
Humoral immune responses induced by tau294–305 epitope to hepatitis B core immunodominant region (T294-HBc) virus-like particle (VLP) vaccine. a Schematic diagram of the time points for treating Tau.P301S mice (5 months old) (n = 7). b The antibody titer induced by T294-HBc VLPs. Sera from immunized mice were serially diluted from 1:100 to 1:819,200 in twofold dilution steps and tested in duplicates by enzyme-linked immunosorbent assay (ELISA) against mis-disordered tau (151–391/2N4R). c The isotypes of vaccine-induced antibodies. The diluted sera were added to mis-disordered tau (151-391/2N4R)-coated plate and followed by adding HRP–conjugated secondary antibodies. d Different binding of serum antibodies to mis-disordered tau (151-391/2N4R) and full-length tau 2N4R. Sera from immunized mice were serially diluted from 1:100 to 1:3906250 in fivefold dilution steps and tested in duplicates by ELISA against mis-disordered tau (151-391/2N4R) and full length tau 2N4R, respectively. EC50, Half-maximal effective concentration. (Statistics were analyzed by student’s t-test, *P<0.05). e The binding activity of the antibody elicited by T294-HBc to different tau fragments. The mixture of 200 μM of different tau fragments with diluted sera was added to the mis-disordered tau-coated plate, and then HRP-conjugated goat anti-mouse immunoglobulin G was added. f The binding activity of the antibodies elicited by T294-HBc to the brain tissues of Tau.P301S mice. Brain tissue was detected via IHC using serum from HBc (1:200)- or T294-HBc (1:200)-treated mice. Scale bar is 200 μm