Fig. 2

Sortilin expression pattern in the forebrain of preplaque triple-transgenic Alzheimer’s disease (3×Tg-AD) mice and middle-aged Macaca monkeys. The immunolabeling is visualized with the rabbit sortilin antibody. Framed areas in the left low-magnification panels (a, d, g) are enlarged in the middle (b, e, h) and right (c, f, i) panels. a–c Immunolabeling in a frontal brain section from a 3×Tg-AD mouse at 6 months of age. Neuronal somata and their proximal dendrites are immunolabeled, occurring over cortical layers II–VI (b), the amygdala (a), thalamic nuclei (a), the stratum pyramidale (s.p.) of hippocampal CA1–CA3 regions, and the granule cell layer (GCL) of the dentate gyrus (DG) (a, c). In the monkey neocortex, cellular labeling involving largely pyramidal neurons is present over the gray matter across layers II–VI (d–f), with background reactivity seen in the white matter (WM) (d). In the hippocampal formation (g), pyramidal neurons (g, h) and granule cells of the DG (i) are clearly labeled. The molecular layer (ML) shows neuropil labeling with intensity higher than background (g, i). Abbreviations are as in the Fig. 1 legend, as well as the following: TC Temporal cortex, Ent Entorhinal cortex, Sub Subiculum, Hi Hilus, s.r. Stratum radiatum. Scale bar in (a) = 2 mm, applying to (g), equivalent to 1 mm for (d), 500 μm for (b) and 200 μm for (c, e, f, h, i)