Skip to main content
Fig. 5 | Alzheimer's Research & Therapy

Fig. 5

From: Amyloid β-induced impairments on mitochondrial dynamics, hippocampal neurogenesis, and memory are restored by phosphodiesterase 7 inhibition

Fig. 5

PDE7 inhibition modulates amyloid β (Aβ)-induced effects on mitochondrial fission, and fusion protein levels. a Immunoblots and b densitometric analysis of mitochondrial fission and fusion proteins in SH-SY5Y cells exposed to 1 μM oligomeric Aβ42. The fusion proteins mitofusin1 (Mfn1), mitofusin2 (Mfn2), and optic atrophy 1 (Opa1) were reduced in SH-SY5Y cells exposed to 1 μM oligomeric Aβ42. When cells were co-treated with S14, the expression of all these fusion proteins was recovered. Dynamin-related protein 1 (Drp1) levels were significantly increased in Aβ42-treated SH-SY5Y cells, and those levels were restored after co-administration of 15 μM S14. Data are expressed as mean ± SEM; n = 5–7; *p < 0.05; **p < 0.01; ***p < 0.001. c In the transgenic APP/PS1 mice, the ubiquitination pattern of mitofusins was enhanced compared to wild-type (wt) mice and S14 treatment abolished this ubiquitination. Whereas d Opa1 levels were reduced, e Drp1 expression was increased in the hippocampus of APP/PS1 and wild-type mice. S14 treatment restored these protein levels to those observed in wild-type mice. Data are expressed as mean ± SEM; n = 3–6 mice; *p < 0.05. Statistical significance was assessed by two-way ANOVA followed by Fisher’s post-hoc test for multiple comparisons. Veh vehicle

Back to article page