Fig. 3
From: MH84 improves mitochondrial dysfunction in a mouse model of early Alzheimer’s disease
Brain levels of PGC-1α protein (a) and mRNA (b). Representative western blot assays are included in lower part of a. Band of PGC-1α was located in second place at 100 kDa. Tubulin used as loading control. Expression levels of mRNA were normalized to PGK1 and B2M mRNA expression. Citrate synthase (CS) activity as a marker of mitochondrial content was determined in isolated brain mitochondria using a photometrical assay (c). Animals belonged to three different study groups (wild-type(control), Thy-1 AβPPSL (control), and treatment group Thy-1 AβPPSL (MH84)). Data represent means ± SEM. N = 11 (six females, five males); one-way ANOVA with Tukey’s multiple comparison post test (*p < 0.05, ***p < 0.001 against wild-type(control); ++p < 0.01, +p < 0.05 against Thy-1 AβPPSL (control)). PGC-1α peroxisome proliferator-activated receptor-γ coactivator alpha, AβPP beta-amyloid precursor protein