Fig. 4

Immunization with 43D monoclonal antibody blocks Alzheimer’s disease abnormally hyperphosphorylated Tau (AD p-Tau) seeding and inhibits the spread of neurofibrillary pathology in triple-transgenic Alzheimer’s disease (3 × Tg-AD) mice. AD p-Tau (0.35 μg in 2 μl of saline) isolated from AD cerebral cortex was unilaterally injected into the right hippocampus of 11- to 12-month-old female 3 × Tg-AD mice or saline as a vehicle control. The mice were perfused at 7.5 weeks after AD p-Tau injection, and coronal sections were immunostained with monoclonal antibodies AT8 and PHF-1. a Representative image of AT8 staining of the contralateral and ipsilateral CA1 areas of the brain. High-magnification images are shown as insets. b The number of tangles (AT8-positive somatodendritic profiles) was counted from 22 to 25 serial coronal sections per mouse (every fourth section of the brain) at × 10 magnification. c Representative image of PHF-1 staining in the contralateral and ipsilateral CA1 areas. High-magnification images are shown as insets. d The number of tangles (PHF-1-positive somatodendritic profiles) were counted from 22 to 25 serial coronal sections per mouse (every fourth section of the brain) at × 10 magnification. Saline/43D (n = 6), AD p-Tau/43D (n = 6), AD p-Tau/IgG (n = 7). *P < 0.05, **P < 0.01