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Fig. 1 | Alzheimer's Research & Therapy

Fig. 1

From: Tau passive immunization blocks seeding and spread of Alzheimer hyperphosphorylated Tau-induced pathology in 3 × Tg-AD mice

Fig. 1

Study design and characterization of Alzheimer’s disease abnormally hyperphosphorylated Tau (AD P-Tau). a Female triple-transgenic Alzheimer’s disease (3 × Tg-AD) mice were immunized with 43D or as a control with mouse immunoglobulin G (IgG) (i.v., 15 μg in 200 μl of saline) once weekly for 6 weeks. AD p-Tau (0.35 μg in 2.5 μl of saline) isolated from AD cerebral cortex was unilaterally injected into right hippocampus of 11- to 12-month-old 3 × Tg-AD mice on the day of the second dose of immunization. Saline/43D (n = 6), AD p-Tau/43D (n = 6), and AD p-Tau/IgG (n = 7). b Quantities of 5 μg of AD p-Tau (lane 1), 2.5 μg of AD p-Tau (lane 2), and 7 μg of AD brain extract (15,000 × g) were loaded, and blots were developed with PHF-1 antibody. c Quantities of 23 μg of AD p-Tau (lane 1) and 15 μg of N2a cell lysate as a positive control (lane 2) were loaded, and blots were developed with transactive response DNA-binding protein 43 (TDP-43) antibody. d Quantities of 23 μg of AD p-Tau (lane 1) and 0.1 μg of synthetic amyloid-β (Aβ) (lane 2) were loaded, and blots were developed with 4G8 antibody

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