Fig. 4
From: Novel botanical drug DA-9803 prevents deficits in Alzheimer’s mouse models
Calcium overload in DA-9803-treated mice is significantly decreased after 8 weeks of treatment. a, b Two-photon images of cortical neurites, pseudocolored according to neuronal [Ca2+] concentration and intensity, in vehicle-treated (a) and DA-9803-treated (b) mice. Red neurites exhibit elevated levels of calcium, blue neurites exhibit normal calcium levels. Yellow arrows denote overloaded neurites. c, d Histograms of neurite YC3.6 ratios for APP/PS1 mice in the vehicle (c) and DA-9803 (d) conditions. n = 361 neurites in six mice in vehicle condition. n = 290 neurites in five mice in DA-9803 condition. e Percentage of neurites exhibiting calcium overload across conditions after 8 weeks of treatment. Scale bar, 10 μm. Mean ± SEM. *p < 0.05. YFP yellow fluorescent protein, CFP cyan fluorescent protein