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Fig. 4 | Alzheimer's Research & Therapy

Fig. 4

From: Anti-β-sheet conformation monoclonal antibody reduces tau and Aβ oligomer pathology in an Alzheimer’s disease model

Fig. 4

Molecular integrity and Alzheimer’s disease (AD) pathology: recognition of the CaptureSelect-purified GW-23B7 infused into old triple-transgenic mice. a Immunoblot showing protein stain with μ and kappa reactivity around the 1000 kDa position of an intact pentameric immunoglobulin M kappa chain (IgMκ) (lane 1). b Immunoblot of paired helical filaments (PHFs) and PHF protein kinase A purified from a human AD brain (lanes A and B) detected using a PHF-1 commercial antibody specific for hyperphosphorylated tau or using GW-23B7. Right panel shows immunohistochemical recognition by GW-23B7 of intra- and extracellular pathology-associated structures on a human AD brain section. c Immunoblots comparing reactivity to Aβ1–42 peptide and Aβ1–42 oligomerized (lanes C and D) by the commercial anti-Aβ 4G8/6E10 antibodies or GW-23B7; right panel shows colocalization on a human AD brain section of punctuated or strong precipitated material recognized by GW-23B7 (black stain) within amyloid plaques of Aβ detected by 4G8/6E10-specific antibodies (red stain)

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Alzheimer's Research & Therapy

ISSN: 1758-9193