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Fig. 7 | Alzheimer's Research & Therapy

Fig. 7

From: Neurons derived from sporadic Alzheimer’s disease iPSCs reveal elevated TAU hyperphosphorylation, increased amyloid levels, and GSK3B activation

Fig. 7

Effect of hydrogen peroxide (H2O2) and amyloid-β1–42 (Aβ1–42) oligomer treatment on neuronal viability. a Viability of induced pluripotent stem cell (iPSC)-derived neurons from control individuals (ctrl-1–ctrl-4), patients with early-onset familial Alzheimer’s disease (fAD) (fAD-1–fAD-4), and patients with sporadic Alzheimer’s disease (sAD) (sAD-1–sAD-6) at day 28 of terminal differentiation (TD28) and TD56 after 24 h of treatment with 30 μM H2O2 (left panel) and 60 μM H2O2 (right panel). b Neuronal survival of control and Alzheimer’s disease (AD) clones at TD28 and TD56 cultured 24 h in the presence of 5 μM Aβ1–42 oligomer solution. Neuronal survival was represented as a percentage of control. Viability of the cultures following H2O2 and Aβ treatment was assessed using the CellTiter-Glo® Luminescent Cell Viability Assay. Values are presented as the mean ± SEM (n = 3). Dunnett’s test was performed to evaluate the significance of groups compared with control at the same time point of differentiation (*p < 0.05)

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