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Fig. 5 | Alzheimer's Research & Therapy

Fig. 5

From: Neurons derived from sporadic Alzheimer’s disease iPSCs reveal elevated TAU hyperphosphorylation, increased amyloid levels, and GSK3B activation

Fig. 5

Western blot analysis of total TAU and phosphorylated TAU (pTAU) protein. a Schematic representation of human TAU isoform (441 amino acids) with the functional projection and microtubule-binding domains. Projection domains including a proline-rich region and N-terminal part interact with cytoskeletal elements and are involved in signal transduction. Microtubule-binding domains with a C-terminal part regulate the microtubule polymerization and bind to proteins such as presenilin 1 (PSEN1). Epitopes of pTAU antibodies analyzed in this study are indicated on the scheme. bf Densitometric analysis of TAU phosphorylated at different epitopes: S262, S396, S202/T205, T181, and S400/T403/S404. All samples were analyzed at day 70 of terminal differentiation. The amount of pTAU relative to total TAU levels in the lysates was measured. GAPDH as the loading control was used to normalize the data. All values are the mean ± SEM (n = 3). Dunnett’s test was performed to evaluate the significance of groups compared with control (*p < 0.05)

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