Fig. 5
Amyloid-β (Aβ)4–x peptides in transgenic Alzheimer’s disease (AD) mouse models. Using antibody D3E10 against Aβ42, abundant extracellular deposits were detected in 10-month-old 5XFAD mice by immunohistochemistry (a, a′). In contrast, the Aβ4–x-specific antibody 029-2 showed a strongly reduced staining pattern that was restricted to amyloid cores (b, b′). Staining with an amyloid precursor protein (APP) antibody decorated only dystrophic neurites (c) surrounding the Aβ4–x-positive plaque core (d, e). The restricted Aβ4–x immunoreactivity within plaque cores was further demonstrated using double-labeling of D3E10 (f) and 029-2, which decorated only the central plaque core (g, h). A similar staining pattern to that in 5XFAD mice was observed in 8-month-old APP/PS1KI mice, as exemplified in the hippocampus (D3E10 [i] and 029-2 [j]). Scale bar = 1000 μm (a, b); 60 μm (a′, b′, f–h); 33 μm (c–e); 100 μm (i, j)