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Fig. 7 | Alzheimer's Research & Therapy

Fig. 7

From: Dietary arachidonic acid increases deleterious effects of amyloid-β oligomers on learning abilities and expression of AMPA receptors: putative role of the ACSL4-cPLA2 balance

Fig. 7

Modifications of brain cytosolic phospholipase A2 (cPLA2) and neuron-specific acyl-coenzyme A synthetase 4 (nACSL4) proteins induced by arachidonic acid-enriched (ARA) diet and amyloid-β peptide 42 (Aβ42). Immediately after the probe test, mice were killed, and homogenates were prepared from the cortex and hippocampus. Representative immunoblots of cortical (a and c) and hippocampal (b and d) phosphorylated cytosolic phospholipase A2 (P-cPLA2) and cPLA2 (a and b) and nACSL4 (c and d) proteins from oleic acid-enriched (OLE) or ARA diet mice after NaCl or Aβ42 injections are shown. Densitometric analyses were performed to determine signal intensities normalized to β-tubulin for cPLA2 and nACSL4 and normalized to cPLA2 for P-cPLA2, and data are expressed as percentages of control OLE mice injected with NaCl (* p < 0.05, comparing the four groups of mice). In (a) and (b), P-cPLA2/cPLA2 density is shown by the black and white scales (y-axis on the left), and cPLA2/β-tubulin is shown by the gray circles (y-axis on the right). Results are shown as mean ± SEM of immunoblots performed for all animals (OLE groups, n = 4; ARA groups, n = 6)

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Alzheimer's Research & Therapy

ISSN: 1758-9193