Fig. 7

Effect of silencing and overexpression of MAO-B on Aβ production and Notch processing in HEK293 cells and HEPG2 cells. a HEK-APP cells were transfected by lipofection with two different siRNAs targeting MAO-B and after 24 h the levels of Aβ40 and Aβ42 in the conditioned medium were measured by ELISA. b HEK cells overexpressing Notch lacking the ectodomain (Notch ΔE) were transfected with siRNA targeting MAO-B and after 3 days of transfection the cells were lysed, subjected to WB analysis and the NICD levels were determined from CCD camera images of the WB. A typical WB is shown, n = 3. c Effect of overexpression of MAO-B and C99 in HEPG2 cells on Aβ production. HepG2 cells were coinfected with BacMam-C99 and BacMam-MAO-B. Levels of Aβ40 secreted into the conditioned medium were measured by a HTRF assay. Data shown represent mean values ± SD (n = 8). Significance was determined using two-tailed, two-sample unequal variance t test. ***p < 0.001. Aβ amyloid β-peptide, MOI multiplicity of infection, NICD Notch intracellular domain, siRNA small interfering RNA