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Fig. 5 | Alzheimer's Research & Therapy

Fig. 5

From: Monoamine oxidase B is elevated in Alzheimer disease neurons, is associated with γ-secretase and regulates neuronal amyloid β-peptide levels

Fig. 5

Proximity ligation assay (PLA) to visualize the PS1/MAO-B and γ-secretase/MAO-B interactions in mouse brain. Female adult mouse brains were fixed in formalin, and 10-μm sections were cut using a cryostat and stored at −20 °C prior to PLA assay. a Cross-section of a mouse brain showing the regions chosen for PLA imaging: cortex (blue squares) and hippocampal CA1 (red squares). be PLA in mouse hippocampal CA1 to visualize the association between PS1 and MAO-B (using a PS1 and an MAO-B antibody) and between active γ-secretase and MAO-B (using GTB and an MAO-B antibody) as well as negative controls (only MAO-B antibody and competition in the presence of L-685,458). fi PLA in mouse cortex (with the same antibody and GTB combinations as in b–e). Insets are enlarged parts of the original images to clearly visualize the PLA signals (note that the small size of PLA signals makes it difficult to see them on printouts). j Quantification of the PLA signals in hippocampal CA1 and cortex for the PS1/MAO-B association. k Quantification of the PLA signals in hippocampal CA1 and cortex for the association between mature γ-secretase and MAO-B. Quantification of the number of PLA signals was done using the Duolink image tool. Mean values ± SE from at least three images in 10 experiments (traditional PLA) or three experiments (GTP-PLA) are shown (Color figure online)

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