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Fig. 6 | Alzheimer's Research & Therapy

Fig. 6

From: Prevention of dendritic and synaptic deficits and cognitive impairment with a neurotrophic compound

Fig. 6

Prevention of dendritic and synaptic deficits and rescue of CREB activity loss by P021 in 3 × Tg-AD mice at 18 month posttreatment. After 18 months, P021- (Tg-AD-P021) and vehicle-treated (Tg-AD vh) mice were euthanized and expressions and/or levels of synapsin 1, NR1, synaptophysin, MAP2, phosphor- (pCREB) and total CREB, synapsin 1, GluR1, and PSD 95 were studied immunohistochemically or by Western blot. a Immunohistochemical analysis of synapsin 1 expression in CA1 (stratum radiatum and stratum pyramidae) and b NR1in parietal cortex (all six layers). c Quantification of immunohistochemical staining shown in a and b. d Western blots of synaptic proteins in the whole cortex and e the whole hippocampus. f Relative quantification of the blots. All blots were normalized against the loading control GAPDH. The level and the expression of several synaptic markers were deficient in the 3 × Tg-AD-vh mice and P021 was able to rescue this deficit. *p < 0.05, ***p < 0.001, by one-way ANOVA post-hoc test. Scale bar = 100 μm. Wild-type (WT), n = 5; Tg-AD-vh, n = 5; Tg-AD-P021, n = 5. Each maximum projection picture to be analyzed was converted to greyscale and each area to be analyzed was selected, the threshold was adjusted, and the mean pixel intensity of the region of interest was quantified. Quantification is shown as mean ± SD

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