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Fig. 2 | Alzheimer's Research & Therapy

Fig. 2

From: Prevention of dendritic and synaptic deficits and cognitive impairment with a neurotrophic compound

Fig. 2

Dendritic and synaptic deficits in 3 × Tg-AD mice at 3 months of age. Expressions of dendritic and synaptic markers were studied immunohistochemically in 3-month-old female 3 × Tg-AD fed a vehicle diet (Tg-AD vh) and control wild-type (WT) mice. a Immunohistochemical staining of MAP2 in the CA1 (stratum radiatum and stratum pyramidae) and dentate gyrus (DG; entire area); b synaptophysin in CA3 (stratum oriens, stratum radiatum, and stratum pyramidae) and DG; c PSD-95 in the parietal cortex (all six layers); d NR1 in the parietal cortex; e quantification of immunohistochemical staining by mean pixel intensity. At the age of 3 months the 3 × Tg-AD mice show a decrease in the level of expression of several synaptic proteins compared to WT. *p < 0.05, **p < 0.01, by t test. Scale bars = 100 μm. WT, n = 5; 3 × Tg-AD, n = 5. Each maximum projection picture to be analyzed was converted to grayscale and each area to be analyzed was selected, the threshold was adjusted, and the mean pixel intensity of the region of interest was quantified. Quantification is shown as mean ± SD

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