TY - JOUR AU - Vanderstichele, Hugo AU - Demeyer, Leentje AU - Janelidze, Shorena AU - Coart, Els AU - Stoops, Erik AU - Mauroo, Kimberley AU - Herbst, Victor AU - François, Cindy AU - Hansson, Oskar PY - 2017 DA - 2017/06/06 TI - Recommendations for cerebrospinal fluid collection for the analysis by ELISA of neurogranin trunc P75, α-synuclein, and total tau in combination with Aβ(1–42)/Aβ(1–40) JO - Alzheimer's Research & Therapy SP - 40 VL - 9 IS - 1 AB - The pathophysiology of neurodegeneration is complex. Its diagnosis requires an early identification of sequential changes in several hallmarks in the brains of affected subjects. The presence of brain pathology can be visualized in the cerebrospinal fluid (CSF) by protein profiling. It is clear that the field of Alzheimer’s disease (AD) will benefit from an integration of algorithms including CSF concentrations of individual proteins, especially as an aid in clinical decision-making or to improve patient enrolment in clinical trials. The protein profiling approach requires standard operating procedures for collection and storage of CSF which must be easy to integrate into a routine clinical lab environment. Our study provides recommendations for analysis of neurogranin trunc P75, α-synuclein, and tau, in combination with the ratio of β-amyloid Aβ(1–42)/Aβ(1–40). SN - 1758-9193 UR - https://doi.org/10.1186/s13195-017-0265-7 DO - 10.1186/s13195-017-0265-7 ID - Vanderstichele2017 ER -