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Fig. 5 | Alzheimer's Research & Therapy

Fig. 5

From: Sorting nexin-4 regulates β-amyloid production by modulating β-site-activating cleavage enzyme-1

Fig. 5

Sorting nexin-4 (SNX4) interacts with β-site amyloid precursor protein-cleaving enzyme 1 (BACE1). a HEK293 cells were cotransfected with BACE1-hemagglutinin (BACE1-HA) and either mock green fluorescent protein (mock-GFP) or SNX4-GFP. HEK293 cell lysates were coimmunoprecipitated (IP) with HA antibodies, followed by Western blotting (WB) against HA, GFP, and major histocompatibility complex class I (MHC1) antibodies to assess their interaction. b Mouse cortical neurons were cotransfected with BACE1-HA and either mock-GFP or SNX4-GFP. Cell lysates were coimmunoprecipitated with HA antibodies, followed by Western blotting against HA, GFP, and MHC1 antibodies to assess their interaction. c Mouse brain lysates were coimmunoprecipitated with BACE1 antibodies, followed by Western blotting against BACE1 or SNX4 antibodies to assess their endogenous interaction. d HeLa cells were cotransfected with BACE1-HA and either mock-GFP or SNX4-GFP. Immunocytochemistry was performed using an anti-HA antibody (red). Scale bar = 20 μm. e Cortical neurons were cotransfected with BACE1-HA and either mock-GFP or SNX4-GFP. Immunocytochemistry was performed using an anti-HA antibody (red). Scale bar = 20 μm

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