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Table 3 Summary of average enzymes per bead for prototype assay calibration using Quanterix standard for assay evaluation and clinical test batches

From: A digital enzyme-linked immunosorbent assay for ultrasensitive measurement of amyloid-β 1–42 peptide in human plasma with utility for studies of Alzheimer’s disease therapeutics

 

AEB

Theoretical concentration, pg/ml

0.023

0.069

0.206

0.617

1.85

5.6

16.7

50.0

 Run 1

0.006

0.008

0.010

0.023

0.112

0.794

5.951

27.615

 Run 2

0.005

0.006

0.008

0.018

0.086

0.692

5.214

23.725

 Run 3

0.005

0.007

0.009

0.018

0.089

0.703

5.774

29.431

 Run 4

0.006

0.006

0.008

0.020

0.102

0.720

5.665

28.302

 Run 5

0.005

0.006

0.008

0.018

0.096

0.720

5.603

28.131

 Run 6

0.006

0.007

0.009

0.019

0.089

0.624

5.082

27.607

 Run 7

0.011

0.012

0.014

0.025

0.091

0.618

4.674

25.809

 Run 8

0.007

0.009

0.011

0.015

0.107

0.580

5.454

24.691

 Run 9

0.007

0.009

0.010

0.018

0.084

0.608

5.409

24.587

N (total number of runs)

9

9

9

9

9

9

9

9

Inter-assay mean

0.006

0.008

0.010

0.019

0.095

0.673

5.425

26.655

SD

0.00

0.00

0.00

0.00

0.01

0.07

0.39

2.00

CV%

26.4

24.8

19.1

15.4

10.4

10.3

7.2

7.5

  1. AEB Average enzyme per bead, CV% Coefficient of variation percentage
  2. In runs 6–8, calibration curves prepared using both Quanterix and Fujirebio standards were included