Fig. 8
DAPT, an inhibitor of Notch1 signaling, blocked the neuroprotective effects of melatonin against Aβ1–42 in hippocampal neurons. a Representative images of immunofluorescent staining for βIII-tubulin. b Statistical analysis of immunofluorescent staining for βIII-tubulin. c Representative images of immunofluorescent staining for MAP2. d Statistical analysis of immunofluorescent staining for MAP2. Scale bar = 100 μm. **p < 0.01 compared with control cells; ## p < 0.01 compared with Aβ1–42-treated cells; ^^ p < 0.01 compared with melatonin-treated cells; @@ p < 0.01 compared with Aβ1–42 and melatonin-treated cells. Ctrl control cells, Aβ Aβ1–42-treated cells, Mel melatonin-treated cells, Aβ + Mel Aβ1–42 and melatonin-treated cells, Aβ + Mel + D Aβ1–42, melatonin, and DAPT-treated cells, DAPT DAPT (a γ-secretase inhibitor)-treated cells, DAPI 4′,6-diamidino-2-phenylindole