Fig. 4

Modulation of γ-secretase processing of APLP1 in dogs. APLP1 was immunoprecipitated from cerebrospinal fluid collected from dogs prior to and 4, 8 and 24 hours after receiving 20 and 80 mg/kg of the γ-secretase modulator E2012. Using MALDI-TOF/TOF, full mass spectra were recorded in which the intensity of the peak corresponding to a peptide was normalized against the intensity of all the peaks measured in the spectrum. Shown is the percent deviation from the sample collected prior to dosing (baseline) for the peptides a APLP1β17, b APLP1β18, c APLP1β21, d APLP1β22, e APLP1β25, f APLP1β27 and g APLP1β28. The measurements are shown as the mean (n = 7) with 95 % confidence interval. Significant dose effects between time intervals were determined using Friedman test combined with Dunn’s test for multiple comparisons and are indicated as *p < 0.05 and **p < 0.01. APLP1 Amyloid-like protein 1