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Table 4 Raw data for Figure 4 : mean amyloid-beta oligomer values in serial dilutions of AD-TBS extracts from nine brains using the 1C22/3D6B oligomer-specific ELISA

From: A highly sensitive novel immunoassay specifically detects low levels of soluble Aβ oligomers in human cerebrospinal fluid

Dilutions AD1 (pg/ml) AD2 (pg/ml) AD3 (pg/ml) AD4 (pg/ml) AD5 (pg/ml) AD6 (pg/ml) AD7 (pg/ml) AD8 (pg/ml) AD9 (pg/ml)
1:2,000 35.0 ± 18.7 36.8 ± 6 SAT 15.5 ± 0.3 21.5 ± 0.8 SAT 8.7 ± 0.4 42.9 ± 5.0 4.7 ± 0.8
1:4,000 31.0 ± 4.8 17.7 ± 1.4 SAT 9.2 ± 0.4 7.1 ± 0.6 20.8 ± 0.05 3.2 ± 0.5 15.0 ± 0.8 2.9 ± 0.3
1:8,000 12.9 ± 1.6 8.0 ± 0.9 SAT 4.9 ± 0.2 4.9 ± 0.6 13.0 ± 1.16 2.2 ± 0.8 9.9 ± 0.3 1.3 ± 0.2
1:16,000 7.0 ± 0.5 5.0 ± 0.3 SAT 4.0 ± 0.5 2.2 ± 0.4 6.1 ± 0.31 0.9 ± 0.1 4.6 ± 0.1 0.8 ± 0.2
1:32,000 3.5 ± 0.3 2.9 ± 0.6 37.5 ± 2.2 2.5 ± 0.5 1.1 ± 0.1 3.6 ± 0.42 0.4 ± 0.1 2.5 ± 0.1 0.3 ± 0
1:64,000 2.0 ± 0.2 1.4 ± 0.1 17.1 ± 1.2 1.6 ± 0.1 0.6 ± 0.1 1.3 ± 0.1 ND 1.4 ± 0.2 0.4 ± 0
1:128,000 1.2 ± 0.3 0.9 ± 0.2 10.0 ± 1.4 1.4 ± 0.1 ND ND 0.4 0.6 ± 0.1 ND
1:256,000 1.2 ± 0.4 0.9 ± 0.1 4.6 ± 0.3 1.0 ± 0.2 ND ND ND ND ND
AD-TBS (1:2,000) after 1282 + AW7 immunodepletion N/A N/A N/A N/A ND ND 1.4 ± 1.5 0.6 ± 0.1 0.4 ± 0.1
  1. AD, Alzheimer’s disease; ELISA, enzyme-linked immunosorbent assay; SAT, amyloid-beta oligomer values are higher than the highest loaded standard (50 pg/ml); ND, amyloid-beta oligomer values are too low to be detectable; TBS, Tris-buffered saline (20 mM Tris–HCl, pH 7.4, containing 150 mM NaCl).