|
Dilutions
|
AD1 (pg/ml)
|
AD2 (pg/ml)
|
AD3 (pg/ml)
|
AD4 (pg/ml)
|
AD5 (pg/ml)
|
AD6 (pg/ml)
|
AD7 (pg/ml)
|
AD8 (pg/ml)
|
AD9 (pg/ml)
|
|---|
|
1:2,000
|
35.0 ± 18.7
|
36.8 ± 6
|
SAT
|
15.5 ± 0.3
|
21.5 ± 0.8
|
SAT
|
8.7 ± 0.4
|
42.9 ± 5.0
|
4.7 ± 0.8
|
|
1:4,000
|
31.0 ± 4.8
|
17.7 ± 1.4
|
SAT
|
9.2 ± 0.4
|
7.1 ± 0.6
|
20.8 ± 0.05
|
3.2 ± 0.5
|
15.0 ± 0.8
|
2.9 ± 0.3
|
|
1:8,000
|
12.9 ± 1.6
|
8.0 ± 0.9
|
SAT
|
4.9 ± 0.2
|
4.9 ± 0.6
|
13.0 ± 1.16
|
2.2 ± 0.8
|
9.9 ± 0.3
|
1.3 ± 0.2
|
|
1:16,000
|
7.0 ± 0.5
|
5.0 ± 0.3
|
SAT
|
4.0 ± 0.5
|
2.2 ± 0.4
|
6.1 ± 0.31
|
0.9 ± 0.1
|
4.6 ± 0.1
|
0.8 ± 0.2
|
|
1:32,000
|
3.5 ± 0.3
|
2.9 ± 0.6
|
37.5 ± 2.2
|
2.5 ± 0.5
|
1.1 ± 0.1
|
3.6 ± 0.42
|
0.4 ± 0.1
|
2.5 ± 0.1
|
0.3 ± 0
|
|
1:64,000
|
2.0 ± 0.2
|
1.4 ± 0.1
|
17.1 ± 1.2
|
1.6 ± 0.1
|
0.6 ± 0.1
|
1.3 ± 0.1
|
ND
|
1.4 ± 0.2
|
0.4 ± 0
|
|
1:128,000
|
1.2 ± 0.3
|
0.9 ± 0.2
|
10.0 ± 1.4
|
1.4 ± 0.1
|
ND
|
ND
|
0.4
|
0.6 ± 0.1
|
ND
|
|
1:256,000
|
1.2 ± 0.4
|
0.9 ± 0.1
|
4.6 ± 0.3
|
1.0 ± 0.2
|
ND
|
ND
|
ND
|
ND
|
ND
|
|
AD-TBS (1:2,000) after 1282 + AW7 immunodepletion
|
N/A
|
N/A
|
N/A
|
N/A
|
ND
|
ND
|
1.4 ± 1.5
|
0.6 ± 0.1
|
0.4 ± 0.1
|
- AD, Alzheimer’s disease; ELISA, enzyme-linked immunosorbent assay; SAT, amyloid-beta oligomer values are higher than the highest loaded standard (50 pg/ml); ND, amyloid-beta oligomer values are too low to be detectable; TBS, Tris-buffered saline (20 mM Tris–HCl, pH 7.4, containing 150 mM NaCl).
