A highly sensitive novel immunoassay specifically detects low levels of soluble Aβ oligomers in human cerebrospinal fluid

Table 3 Comparison of sensitivities (LLoQ) of the 1C22/3D6B amyloid-beta oligomer assay using the MSD versus Erenna platforms

ELISA platform	ADDLs (pg/ml)	(S26C) ₂ (pg/ml)	Dityrosine (pg/ml)
Erenna	0.15	40.6 ± 9.3	375 ± 84.6
MSD	18.7	187 ± 54	601 ± 107

LLoQ is the lowest back-interpolated standard that provides a signal two-fold greater than background with a coefficient of variance <20%. ADDL, amyloid-beta-derived diffusible ligand; ELISA, enzyme-linked immunosorbent assay; LLoQ, lower limit of reliable quantification. Erenna platform (Singulex, Alameda, CA, USA) and MSD Multi-Array (Meso Scale Discovery, Gaithersburg, MD, USA).

ISSN: 1758-9193