Figure 2
From: Quantitative neuropathological assessment to investigate cerebral multi-morbidity
Digital quantification in a brain section using alpha-smooth muscle actin (α -SMA) immunohistochemistry which labels arterioles. Actual immunohistochemical stain is brown (A) whereas the digital detection of those markers is pseudocolored green (B) after digital analyses were run. This is the basis for further in silico analyses of the blood vessel morphology. Scale bars, 100 μm. (C) A sample of the data that are collected from each analysis. Note that aspects of the blood vessel lumen, in addition to the lumen wall, are measured in a systematic way.