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Table 5 Semiquantitative rating of immunoblotting of HSP27 and comparison with immunohistochemistry a

From: Expression of one important chaperone protein, heat shock protein 27, in neurodegenerative diseases

  Approximately 25-kDa band Approximately 50-kDa band IHC rating scores
Case WL LS TX SARK UF WL LS TX SARK UF F-N F-GG F-WG F-Astro
1 ++++ ++ + ++ +/- +/- +/- - - - 3 3 2 3
2 +++ +++ ++ +++ +++ + ++ + - - 3 3 3 3
3 + +/- - - +/- ++ +++ + - ++ 2 3 4 4
4 +++ ++ + +++ ++ +/- ++ +/- - +/- nd nd nd nd
5 - - - ++ - - +/- - - - 1 1 1 2
  1. a+/++/+++ of bands; +/- very faint bands; - no bands at all; F-Astro, Astrocytes of frontal cortex; F-GG, Grey matter glial cells of frontal cortex; F-N, Neurons of frontal cortex; F-WG, White matter glial cells of frontal cortex; IHC, Immunohistochemistry; LS, Low salt fraction; nd, not done; SARK, Sarkosyl fraction; TX, Triton X-100 fraction; UF, Urea fraction; WL, Whole protein fractions.