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Table 5 Semiquantitative rating of immunoblotting of HSP27 and comparison with immunohistochemistry a

From: Expression of one important chaperone protein, heat shock protein 27, in neurodegenerative diseases

 

Approximately 25-kDa band

Approximately 50-kDa band

IHC rating scores

Case

WL

LS

TX

SARK

UF

WL

LS

TX

SARK

UF

F-N

F-GG

F-WG

F-Astro

1

++++

++

+

++

+/-

+/-

+/-

-

-

-

3

3

2

3

2

+++

+++

++

+++

+++

+

++

+

-

-

3

3

3

3

3

+

+/-

-

-

+/-

++

+++

+

-

++

2

3

4

4

4

+++

++

+

+++

++

+/-

++

+/-

-

+/-

nd

nd

nd

nd

5

-

-

-

++

-

-

+/-

-

-

-

1

1

1

2

  1. a+/++/+++ of bands; +/- very faint bands; - no bands at all; F-Astro, Astrocytes of frontal cortex; F-GG, Grey matter glial cells of frontal cortex; F-N, Neurons of frontal cortex; F-WG, White matter glial cells of frontal cortex; IHC, Immunohistochemistry; LS, Low salt fraction; nd, not done; SARK, Sarkosyl fraction; TX, Triton X-100 fraction; UF, Urea fraction; WL, Whole protein fractions.