Figure 1
From: PLD3 is accumulated on neuritic plaques in Alzheimer’s disease brains
Constitutive expression of PLD3 mRNA in human neural cells. mRNA expression was studied by reverse transcription (RT)-polymerase chain reaction in human tissues and cultured cells. (a) PLD1, (b) PLD2, (c) PLD3, (d) PLD4, (e) PLD5, (f) PLD6, and (g) glyceraldehyde-3-phosphate dehydrogenase (G3PDH). Lane 1, frontal cortex of the human cerebrum (CBR) with inclusion of the RT step; lane 2, CBR without inclusion of the RT step; lane 3, astrocytes (AS); lane 4, neuronal progenitor (NP) cells; lane 5, NTera2 teratocarcinoma-derived neurons (NTera2N); lane 6, SK-N-SH neuroblastoma; lane 7, IMR-32 neuroblastoma; lane 8, U-373MG glioblastoma; lane 9, T98G glioblastoma; lane 10, HMO6 immortalized microglia. cDNA was amplified by PCR for 35 cycles, except for G3PDH that was amplified for 28 cycles. PLD, phospholipase D.