Figure 4

Coimmunization suppressed the expression of inflammatory cytokines in the brains of amyloid precursor protein 695 transgenic mice. Protein lysates were prepared from the brains of wild-type (WT), amyloid protein precursor 695 (APP₆₉₅) and vaccinated mice 7 days after the fifth immunization (n = 5). The expression of cytokines was determined by cytometric bead array. Levels of interferon γ (IFN-γ), tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β) and IL-6 were analyzed, and mean ± SD values are expressed as picograms per 100 milligrams of brain tissue. Statistical analysis was performed using parametric one-way analysis of variance, and t-tests were used to compare two groups. *P < 0.05 compared with APP₆₉₅ mice. co-Aβ42, APP₆₉₅ mice immunized with co-amyloid-β42 vaccine; pro-Aβ42, APP₆₉₅ mice immunized with amyloid-β42 protein vaccine.