Figure 1
Coimmunization against Aβ induces high levels of anti-Aβ42 immunoglobulin G. Serum samples were collected 7 days after the last immunization of C57BL/6 mice (A) and amyloid precursor protein APP695 mice (C), and the anti-Aβ immunoglobulin G (IgG) titer was determined by enzyme-linked immunosorbent assay (ELISA). Mean titers (n = 6) are expressed in log10 units. (B) Immunofluorescent staining of hippocampal and cortical tissue sections of 9-month-old APP/PS1-transgenic mice. The sections were stained with anti-sera from untreated mice (naïve; wild type (WT)), coimmunized mice (co-Aβ42) and protein-immunized mice (pro-Aβ42). Polyclonal anti-Aβ antibody 3552 served as a positive control. Red: secondary antibody; Blue: 4′,6-diamidino-2-phenylindole. All data are presented as mean ± SD. Statistical analysis was performed using parametric one-way analysis of variance, and t-tests were used to compare two groups. The results of one of three independent experiments are shown. *P < 0.05.