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Table 2 Squalene does not reverse the effects of Hexa-PAF upon Aβ 42 trafficking

From: Platelet-activating factor antagonists enhance intracellular degradation of amyloid-β42 in neurons via regulation of cholesterol ester hydrolases

  Concentration of Aβ42, nM
  Cell extracts DRMs Lysosomes
Control 8.7 ± 0.7 8.4 ± 0.6 0.4 ± 0.2
Hexa-PAF 8.5 ± 1.2 2.5 ± 0.9a 3 ± 0.6b
Squalene 8.5 ± 0.9 8 ± 0.4 0.5 ± 0.2
Hexa-PAF + squalene 8.8 ± 0.6 2.8 ± 0.8a 3 ± 0.4b
  1. Neurons were pre-treated with 1 μM Hexa-PAF, 5 μM squalene, or a mixture of Hexa-PAF and squalene and then incubated with conditioned media from 7PA2 cells (7PA2-CM) containing 10 nM Aβ42 for 2 hours. The amounts of Aβ42 in whole cell extracts, detergent-resistant membranes (DRMs) (lipid rafts), and lysosomes were measured by enzyme-linked immunosorbent assay. Values are the mean concentration of Aβ42 ± standard deviation from triplicate experiments performed three times (n = 9). aAmounts of Aβ42 significantly lower than in control cells. bAmounts of Aβ42 significantly higher than in control cells. Hexa-PAF, 1-O-hexadecyl-2-acetyl-sn-glycerol-3-phospho-(N,N,N-trimethyl)-hexanolamine.