| |
Concentration of Aβ42, nM
|
|---|
| |
Cell extracts
|
DRMs
|
Lysosomes
|
|---|
|
Control
|
8.7 ± 0.7
|
8.4 ± 0.6
|
0.4 ± 0.2
|
|
Squalestatin
|
8.7 ± 1
|
2.4 ± 0.6a
|
2.8 ± 0.5b
|
|
Squalene
|
8.5 ± 0.85
|
8 ± 0.4
|
0.5 ± 0.2
|
|
Squalestatin + squalene
|
8.3 ± 0.6
|
7.9 ± 0.9
|
0.3 ± 0.1
|
- Neurons were pre-treated with 200 nM squalestatin, 5 μM squalene, or a mixture of squalestatin and squalene and then incubated with conditioned media from 7PA2 cells (7PA2-CM) containing 10 nM Aβ42 for 2 hours. The concentrations of Aβ42 in whole cell extracts, detergent-resistant membranes (DRMs) (lipid rafts), and lysosomes were measured by enzyme-linked immunosorbent assay. Values are the mean concentration of Aβ42 ± standard deviation from triplicate experiments performed three times (n = 9). aAmounts of Aβ42 significantly lower than in control cells. bAmounts of Aβ42 significantly higher than in control cells.
