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Table 1 Squalene reversed the effects of squalestatin upon Aβ 42 trafficking

From: Platelet-activating factor antagonists enhance intracellular degradation of amyloid-β42 in neurons via regulation of cholesterol ester hydrolases

 

Concentration of Aβ42, nM

 

Cell extracts

DRMs

Lysosomes

Control

8.7 ± 0.7

8.4 ± 0.6

0.4 ± 0.2

Squalestatin

8.7 ± 1

2.4 ± 0.6a

2.8 ± 0.5b

Squalene

8.5 ± 0.85

8 ± 0.4

0.5 ± 0.2

Squalestatin + squalene

8.3 ± 0.6

7.9 ± 0.9

0.3 ± 0.1

  1. Neurons were pre-treated with 200 nM squalestatin, 5 μM squalene, or a mixture of squalestatin and squalene and then incubated with conditioned media from 7PA2 cells (7PA2-CM) containing 10 nM Aβ42 for 2 hours. The concentrations of Aβ42 in whole cell extracts, detergent-resistant membranes (DRMs) (lipid rafts), and lysosomes were measured by enzyme-linked immunosorbent assay. Values are the mean concentration of Aβ42 ± standard deviation from triplicate experiments performed three times (n = 9). aAmounts of Aβ42 significantly lower than in control cells. bAmounts of Aβ42 significantly higher than in control cells.
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Alzheimer's Research & Therapy

ISSN: 1758-9193