Figure 4
Platelet-activating factor (PAF) receptor antagonists altered the distribution of Aβ 42 in neurons. (A) The amount of Aβ42 in detergent-soluble membrane (DSM) (□) or detergent-resistant membrane (DRM) (■) extracts from neurons pre-treated with control medium, 1 μM ginkgolide B, or 1 μM Hexa-PAF and pulsed with conditioned media from 7PA2 cells (7PA2-CM) containing 10 nM Aβ42 for 2 hours. Values are mean ± standard deviation (SD) from triplicate experiments performed four times (n = 12). *Concentration of Aβ42 significantly higher (P <0.05) than in control neurons. (B) Neurons treated with control medium (●) or 1 μM Hexa-PAF (□) and pulsed with 7PA2-CM containing 10 nM Aβ42 for 2 hours. Cell extracts were separated on sucrose density gradients. Values are mean Aβ42 ± SD from triplicate experiments (n = 3). (C) Immunoblot showing the amounts of lysosome-associated membrane protein 1 (LAMP-1) in neurons treated with control medium or 1 μM Hexa-PAF. (D) Neurons were pre-treated with control medium (□), 1 μM Hexa-PAF, 1 μM ginkgolide B, or 1 μM PAF and pulsed with 7PA2-CM containing 10 nM Aβ42 for 2 hours when lysosomes were isolated. Values are mean Aβ42 ± SD from triplicate experiments performed four times (n = 12). *Concentration of Aβ42 significantly higher (P <0.05) than in control neurons. Hexa-PAF, 1-O-hexadecyl-2-acetyl-sn-glycerol-3-phospho-(N,N,N-trimethyl)-hexanolamine.