Figure 4

Effect of consecutive tube transfer on total tau. A) Pilot: data represent one assay. Neat and Tween control CSF T-tau concentration did not decrease significantly with consecutive transfer between tubes. Samples were diluted 1:2. Lines of the same type represent within plate replicates. B) Replication: data represent three assays. Neat CSF T-tau concentration decreased significantly with consecutive transfer between tubes. Tween CSF T-tau was not significantly affected by consecutive transfer between tubes. Samples were diluted 1:4. Lines of the same type represent within and between plate replicates, separate days are not distinguished. CSF, cerebrospinal fluid; T-tau, total tau.