Influence of free or nanoparticulate flurbiprofen on Aβ generation in an in vitro BBB model. 7WD10 cells were co-cultured abluminal with post-confluent bEnd.3 cells in the luminal compartment (A). bEnd.3 cells were treated with the indicated concentrations of free flurbiprofen (B) or PLA-flurbiprofen nanoparticles (C) for 72 hours. Levels of Aβ in the abluminal compartments were measured by an Aβ specific ELISA. The data represent mean ± SEM of three independent experiments. * Statistically significant difference (P <0.05, two-way ANOVA) between Aβ levels of vehicle-treated and drug-treated cells are indicated. Aβ, amyloid-β; ANOVA, analysis of variance; BBB, blood-brain barrier; PLA, polylactide; SEM, standard error of the mean.