Figure 8

Histological analysis of biomarkers in the brain. Top half of Figure: effect of D-Ala2 glucose-dependent insulinotropic polypeptide (GIP) treatment on cell proliferation of 12-month-old APP/PS1 mice and wild type (WT) littermates. Quantification of (A) BrdU-positive cells in the dentate gyrus (DG) of APP/PS1 and WT mice. Data show mean ± standard error of the mean (SEM) of six mice per group (one-way analysis of variance, ANOVA). The micrographs illustrate examples of the DG of WT mice injected with (B) saline and (C) D-Ala²GIP, and APP/PS1 mice injected with (D) saline and (E) D-Ala²GIP peptide, immunohistologically stained against BrdU (scale bar: 210 μm). Bottom half of Figure: effect of D-Ala²GIP treatment on neurogenesis of 12-month-old APP/PS1 mice and WT littermates. Quantification of (A) doublecortin (DCX)-positive cells in the DG of APP/PS1 and WT mice. Data show mean ± SEM of 6 mice per group (one-way ANOVA). The micrographs illustrate examples of the DG of WT mice injected with (B) saline and (C) D-Ala²GIP, and APP/PS1 mice injected with (D) saline and (E) D-Ala²GIP peptide, immunohistologically stained against DCX (scale bar: 210 μm).