Table 2 Primary antibodies utilized for immunohistochemistry and western blot analysis
From: Dystrophic neurites express C9orf72 in Alzheimer's disease brains
Antibody | Supplier | Code | Origin | Antigen utilized for raising antibodies | Concentration used for IHC (μg/ml) | Concentration used for WB (μg/ml) |
|---|---|---|---|---|---|---|
C9orf72 | Santa Cruz Biotechnology (Santa Cruz, CA, USA) | sc-138763 | Rabbit | Peptide spanning amino acid residues 165 to 215 of human C9orf72 | 0.1 | 0.1 |
C9orf72 | Sigma (St. Louis, MO, USA) | HPA023873 | Rabbit | Peptide spanning amino acid residues 110 to 199 of human C9orf72 | 0.72 | 0.1 |
UBQLN1 | Santa Cruz Biotechnology | sc-14652 | Goat | Peptide mapping within an internal region of human UBQLN1 | 1 | 0.2 |
UBQLN2 | Santa Cruz Biotechnology | sc-14658 | Goat | Peptide mapping within an internal region of human UBQLN2 | 2 | NA |
Amyloid beta 11 to 28 | Immunobiological Laboratory (Gunma, Japan) | 10027 (12B2) | Mouse | Human amyloid beta 11 to 28 peptide | 1 | NA |
p62/sequestosome 1 | BD Biosciences (San Jose, CA, USA) | 610832 | Mouse | Peptide spanning amino acid residues 257 to 437 of human p62 | 1 | NA |
Ubiquitin | Santa Cruz Biotechnology | sc-8017 (P4D1) | Mouse | Peptide spanning amino acid residues 1 to 76 of bovine ubiquitin | 0.2 | NA |
PHF-tau | Thermo Scientific (Rockford, IL, USA) | MN1020 (AT8) | Mouse | Partially purified human PHF-tau | 0.25 | 0.2 |
pS409/410 TDP-43 | Cosomo Bio (Tokyo, Japan) | TIP-PTD-M01 | Mouse | Phosphopeptide CMDSKpSpSGWGM | Diluted at 1:500 | NA |
GFAP | Nichirei (Tokyo, Japan) | 422261 (GA5) | Mouse | GFAP purified from swine spinal cord | Prediluted | Further diluted at 1:1,000 |
HSP60 (N-20) | Santa Cruz Biotechnology | sc-1052 | Goat | Peptide mapping in the N-terminus of human HSP60 | NA | 0.1 |