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Table 2 Primary antibodies utilized for immunohistochemistry and western blot analysis

From: Dystrophic neurites express C9orf72 in Alzheimer's disease brains

Antibody Supplier Code Origin Antigen utilized for raising antibodies Concentration used for IHC (μg/ml) Concentration used for WB (μg/ml)
C9orf72 Santa Cruz Biotechnology (Santa Cruz, CA, USA) sc-138763 Rabbit Peptide spanning amino acid residues 165 to 215 of human C9orf72 0.1 0.1
C9orf72 Sigma (St. Louis, MO, USA) HPA023873 Rabbit Peptide spanning amino acid residues 110 to 199 of human C9orf72 0.72 0.1
UBQLN1 Santa Cruz Biotechnology sc-14652 Goat Peptide mapping within an internal region of human UBQLN1 1 0.2
UBQLN2 Santa Cruz Biotechnology sc-14658 Goat Peptide mapping within an internal region of human UBQLN2 2 NA
Amyloid beta 11 to 28 Immunobiological Laboratory (Gunma, Japan) 10027 (12B2) Mouse Human amyloid beta 11 to 28 peptide 1 NA
p62/sequestosome 1 BD Biosciences (San Jose, CA, USA) 610832 Mouse Peptide spanning amino acid residues 257 to 437 of human p62 1 NA
Ubiquitin Santa Cruz Biotechnology sc-8017 (P4D1) Mouse Peptide spanning amino acid residues 1 to 76 of bovine ubiquitin 0.2 NA
PHF-tau Thermo Scientific (Rockford, IL, USA) MN1020 (AT8) Mouse Partially purified human PHF-tau 0.25 0.2
pS409/410 TDP-43 Cosomo Bio (Tokyo, Japan) TIP-PTD-M01 Mouse Phosphopeptide CMDSKpSpSGWGM Diluted at 1:500 NA
GFAP Nichirei (Tokyo, Japan) 422261 (GA5) Mouse GFAP purified from swine spinal cord Prediluted Further diluted at 1:1,000
HSP60 (N-20) Santa Cruz Biotechnology sc-1052 Goat Peptide mapping in the N-terminus of human HSP60 NA 0.1
  1. GFAP, glial fibrillary acidic protein; HSP60, 60 kDa heat shock protein; IHC, immunohistochemistry; NA, not applied; PHF, paired helical filament; TDP-43, TAR DNA-binding protein-43; WB, western blot; UBQLN, ubiquilin.