C9orf72 expression in Alzheimer's disease and non-Alzheimer's disease brains by western blot analysis. Expression of chromosome 9 open reading frame 72 (C9orf72) protein studied in the frozen frontal cortex tissues by western blot analysis. Total protein extract of 15 μg was loaded on each lane. (a) sc-138763. (b) HPA023873. (c) Glial fibrillary acidic protein (GFAP). (d) Hsp60, an internal control for protein loading. Lanes 1 to 7, brains derived from Alzheimer's disease (AD) patients; lanes 8 to 11, brains derived from normal control subjects (non-neurological cause (NC)); lanes 12 to 17, brains derived from amyotrophic lateral sclerosis (ALS) patients; and lanes 18 to 21, brains derived from Parkinson's disease (PD) patients (see Table 1). The position of the molecular weight marker is indicated on the left.