Figure 4
From: Overlapping profiles of Aβ peptides in the Alzheimer's disease and pathological aging brains
Mass spectrometric (MS) analysis following immunoprecipiation of Aβ from 2% SDS extracted lysates of human subjects. 2% SDS lysates of Alzheimer's disease (AD; A, D), pathological aging (PA; B, E) and control (NDC; C, F) subjects were subjected to immunoprecipitation with pull-down by Ab9 (A-C), sequential pull-down with Ab9 and 4 G8 (D-F). Representative MS spectra are shown (A-F). Peaks corresponding to Aβ peptides have been labeled according to m/z. Aβ1-28 was spiked in as an experimental control. The 1-42* peak denotes a possible modified Aβ1-42 species (Aβ1-42 + 16 Da, A-E). Aβ, amyloid β; SDS, sodium dodecyl sulfate.