Hippocampal amyloid plaque numbers do not change when LRP1 is reduced. (A and B) Representative examples of immunohistochemical stains with 6E10 antibody (1:1,000, Covance) for Aβ in APPswe/PS1dE9/LRP1lox/lox mice that were either positive or negative for GFAP-Cre. (C) To quantify amyloid deposits, we stained sections with Thioflavin-S, which produces a sharper distinction between tissue and amyloid plaques (see Additional file 5 Figure S4 for an example) and captured images. The hippocampus was outlined and then the number of amyloid plaques inside the hippocampus was counted manually. A dot plot of amyloid plaque numbers in mice that were Cre+ is compared to the numbers in mice that were Cre-. Animals that were used as breeders are noted. No significant differences between genotypes either Cre+ (n = 12) vs. Cre- (n = 13) groups (P = 0.75) or between breeders (n = 10) vs. non-breeders (n = 15) (P = 0.69) was noted.