The level of LRP1 is significantly lower in the mice expressing GFAP-Cre with LRP1 lox/lox. Nine APPswe/PS1dE9 × GFAP-Cre × LRP1 lox/lox mice with different genotypes were used to determine the levels of LRP1. The SDS-soluble fractions of whole brain lysates (homogenized in PBS) containing an equal amount of total protein (50 μg) was loaded in each lane. Antibody α377 to LRP1 (1:1,000, a gift from Dr. Herz, University of Texas Southwestern Medical Center) was used to detect LRP1 level. The 85 kDa band is the endoproteolytically cleaved mature LRP1. Mouse SOD1 (14 kD bands) was used as endogenous loading control. The ratio of band intensity of LRP1 to SOD1 was quantified, standardized and plotted below. The standard deviations of the measurements are shown by error bars.