Identification of structural determinants on tau protein essential for its pathological function: novel therapeutic target for tau immunotherapy in Alzheimer’s disease
© Kontsekova et al.; licensee BioMed Central Ltd. 2014
Received: 20 March 2014
Accepted: 21 July 2014
Published: 1 August 2014
Pathologically modified tau protein is the main feature of Alzheimer’s disease (AD) and related tauopathies. Therefore, immunotherapies that target mis-disordered tau represent a promising avenue for the disease-modifying treatment of AD. In this report, we present our discovery of (1) a novel target for tau immunotherapy; (2) monoclonal antibody DC8E8, which neutralizes this target; and (3) the results of efficacy studies of DC8E8 in a murine model of tauopathy.
In vitro tau oligomerisation assays were used for the selection of antibodies. The therapeutic efficacy of DC8E8 was evaluated in transgenic mice. The structure of the DC8E8 epitope was determined by X-ray crystallography.
Screening of a panel of monoclonal antibodies for their inhibitory activity in an in vitro pathological tau–tau interaction assay yielded DC8E8, which reduced the amount of oligomeric tau by 84%. DC8E8 recognised all developmental stages of tau pathology in AD human brains, including pretangles and intra- and extracellular tangles. Treatment with DC8E8 in a mouse AD model expressing mis-disordered human tau significantly reduced the amount of insoluble oligomerised tau and the number of early and mature neurofibrillary tangles in the transgenic mouse brains. By using a panel of tau-derived peptides in a competitive enzyme-linked immunosorbent assay, we identified the tau domain essential for pathological tau–tau interaction, which is targeted by DC8E8. The antibody was capable of binding to four highly homologous and yet independent binding regions on tau, each of which is a separate epitope. The X-ray structure of the DC8E8 Fab apo form, solved at 3.0 Å, suggested that the four DC8E8 epitopes form protruding structures on the tau molecule. Finally, by kinetic measurements with surface plasmon resonance, we determined that antibody DC8E8 is highly discriminatory between pathological and physiological tau.
We have discovered defined determinants on mis-disordered truncated tau protein which are responsible for tau oligomerisation leading to neurofibrillary degeneration. Antibody DC8E8 reactive with these determinants is able to inhibit tau–tau interaction in vitro and in vivo. DC8E8 is able to discriminate between the healthy and diseased tau proteome, making its epitopes suitable targets, and DC8E8 a suitable candidate molecule, for AD immunotherapy.
Neurodegenerative foldopathies represent a group of human protein-misfolding disorders that are characterised by a pathological alteration in conformation of a native protein which makes it resistant to degradation and leads to pathological gain and loss of function. These are followed by aggregation of the misfolded proteins into insoluble deposits[1, 2]. One of the most prominent protein-misfolding disorders is Alzheimer’s disease (AD). It was estimated that 35.6 million people lived with dementia worldwide in 2010, and the number of patients with dementia is expected to reach 65 million by 2030 and 115.4 million by 2050. There are no effective treatments available for AD. Therefore, much attention is being directed at the development of approaches that counteract the fundamental pathological processes of the disease[4, 5]. Several classes of drug candidates that have a potential to be disease-modifying drugs are now under development. However, because of unsatisfactory results, many of these clinical trials have been discontinued[4, 6]. Thus, identification of the proper target is of utmost importance for selecting effective AD therapies. There is mounting scientific evidence supporting tau-targeted therapy. (1) Tau neurofibrillary pathology is the major correlate of clinical symptoms in AD[7–9]. (2) Distribution of neurofibrillary pathology defines subtypes of AD with distinct clinical characteristics. (3) Neurofibrillary tangles (NFTs) precede amyloid-β pathology[11–13]. (4) Cortical atrophy measured by magnetic resonance imaging is associated with neurofibrillary pathology. (5) Tau pathology in the absence of amyloid pathology strongly correlates with clinical features in human tauopathies such as progressive supranuclear palsy, corticobasal degeneration, tangle-only dementia, argyrophilic grain disease, frontotemporal dementia and Pick’s disease[15–21]. (6) Tau animal models reproduce neuronal and glial tau pathology leading to the progressive cognitive and/or motor impairment and premature death.
The potential of immunotherapeutic strategies to treat AD is one of the most interesting, unexpected and novel findings over the past decade of dementia research. Currently proposed tau immunotherapeutic approaches would target selectively phosphorylated tau species such as phospho-Ser396/phospho-Ser404[22–24], phospho-Thr231/phospho-Ser235 or abnormally phosphorylated tau species such as phospho-Ser422. In contrast to studies focused on individual phosphorylated tau species, in our present study we developed an immunoproteomic discovery platform that allowed us to identify immunologically targetable common denominators of pathologically mis-disordered tau species that are responsible for pathological tau–tau interaction and thus for the progress of neurofibrillary pathology. Discovery of these regions on tau is a prerequisite for the development of successful tau vaccines that will protect neurons from pathological tau–tau interaction.
We identified such a common denominator, a domain on mis-disordered truncated tau that is essential for propagation of tau pathology, and we created a monoclonal antibody—DC8E8—that disables this function. DC8E8 prevents tau aggregation in vitro and reduces the amount of a wide range of tau oligomers and neurofibrillary pathologies in the brain in transgenic animals. Combined with the ability of DC8E8 to discriminate between healthy and pathological tau with high fidelity, this finding opens a promising avenue to the development of AD treatment.
All experiments were performed in accordance with the Slovak and European Community Guidelines and with the approval of the Ethics Committee of the Institute of Neuroimmunology, Slovak Academy of Sciences (Bratislava, Slovakia).
Preparation of hybridoma cell line producing DC8E8
Balb/c mice were immunised with mis-disordered tau protein 151-391/4R. Harvested immune spleen cells were fused with the mouse myeloma cell line NS0 according to a fusion protocol described previously. Growing hybridoma clones were selected for the production of anti-tau-151-391/4R-specific monoclonal antibodies (mAbs) by enzyme-linked immunosorbent assay (ELISA).
Antibodies used in this study
tau assembly–regulating domains in MTBRa region
truncated mis-disordered tau species
Conformational, requiring tau truncated at 391
Screening of monoclonal antibodies using tau–tau interaction assay
The assay to measure the effect of tau-specific antibodies on pathological tau–tau interactions was set up in phosphate-buffered saline (PBS) containing 20 μM (final concentration) of the tested mis-disordered tau protein (151-391/4R), 5 μM heparin (heparin sodium salt from porcine intestinal mucosa, ≥150 IU/mg, dry basis; Sigma-Aldrich, St Louis, MO, USA) and 12.5 μM (final concentration) thioflavin T (Sigma-Aldrich). Each reaction (80 μl final volume) was incubated for 20 hours at 37°C in sealed black solid polystyrene plates (384 wells; Greiner Bio-One, Monroe, NC, USA). Thioflavin T fluorescence was measured using a microplate fluorometer (Fluoroskan Ascent FL; Thermo Labsystems, Milford, MA, USA) with excitation wavelength of 450 nm, emission at 510 nm and 200-ms measurement time. To determine the effect of mAbs on pathological tau–tau interactions, we added purified antibodies at 20 μM final concentrations to the reactions. The reaction mixtures were incubated at 37°C for 20 hours. Antibody DC51 (recognising an envelope protein of the rabies virus) was used as a mock control.
Western blot analysis of oligomerisation reactions
Mis-disordered tau 297-391/4R was incubated for 1, 4 and 20 hours in either the presence or absence of DC8E8 as described above for the tau–tau interaction assay. At the time points indicated, the reactions were stopped by addition of SDS sample loading buffer. For oligomeric tau analysis, 10 μl of each fibrillisation reaction was electrophoresed and transferred to polyvinylidene fluoride (PVDF) membranes. Subsequently, the membrane was incubated for 1 hour with horseradish peroxidase (HRP)–conjugated DC25 diluted 1:1,000 in PBS. The blot was developed with SuperSignal West Pico Chemiluminescent Substrate (Pierce Biotechnology, Rockford, IL, USA), and the chemiluminescent signals were detected using an LAS3000 imaging system (FUJI Photo Film Co, Tokyo, Japan). The chemiluminescent signal intensities were quantified using AIDA Image Data Analyzer software (Raytest, Straubenhardt, Germany).
Indirect enzyme-linked immunosorbent assay
Microtitre plates (SARSTEDT, Nümbrecht, Germany) were coated overnight at 37°C with human tau protein isoforms 2N4R, 2N3R, and their deletion mutants, and with tau-derived peptides (250 ng/well). After blocking with 1% fat-free dried milk, the plates were washed with PBS-0.05% Tween 20 and incubated with 50 μl/well of DC8E8 hybridoma culture supernatant for 1 hour at 37°C. Bound DC8E8 was detected by polyclonal goat anti-mouse immunoglobulin (Ig)/HRP antibody (Dako, Carpinteria, CA, USA) using chromogenic substrate o-phenylenediamine (Sigma-Aldrich). Absorbance was measured at 492 nm using a Multiskan MCC/340 ELISA plate reader (Thermo Labsystems).
Competitive enzyme-linked immunosorbent assay
ELISA plates (SARSTEDT) were coated overnight at 37°C with 250 ng/well of mis-disordered purified tau 151-391/4R. Peptide competitors (>95% purity; EZBiolab, Carmel, IN, USA) were dissolved in PBS at a final concentration of 1 mM. A 200 μM solution of peptides in PBS/Tween 20 were filled in wells of polypropylene microtitre plates (Greiner Bio-One). The mAb DC8E8 was diluted to a concentration of 0.6 μg/ml (3.8 nM) in PBS, and 60 μl of the diluted antibody solution was mixed with 40 μl of peptide solution in the polypropylene plate. The antibody/peptide mixtures were incubated for 1 hour at 25°C. Subsequently, 50 μl/well of antibody/peptide mixtures were transferred onto mis-disordered tau 151-391/4R-coated ELISA plates (in duplicates) and incubated for 1 hour at 25°C. Bound DC8E8 was detected using polyclonal goat anti-mouse Ig/HRP (Dako) with the chromogenic substrate o-phenylenediamine (Sigma-Aldrich).
Preparation of DC8E8 antigen-binding fragment crystals
DC8E8 was produced by hybridoma cells in serum-free media. After purification on a 5-ml protein G Sepharose column (GE Healthcare Life Sciences, Pittsburgh, PA, USA), DC8E8 antigen-binding fragment (Fab) was produced by partial digestion with papain (papain from Carica papaya; Roche Diagnostics, Indianapolis, IN, USA) and purified by affinity purification in protein A and protein G media and by size-exclusion chromatography as described previously. The Fab was concentrated to 10 mg/ml in 0.01 M Tris-HCl, pH 7.2, 0.05 M NaCl by ultrafiltration and stored at 4°C. Crystallisation was performed using a vapour diffusion technique at 21°C with 0.5- to 1-μl hanging drops in EasyXtal plates (QIAGEN, Valencia, CA, USA). The drops were prepared by mixing equal volumes of protein and precipitant solution. The final concentration of the Fab was 9 mg/ml. After crystallisation screening using Structure Screen 1 (Molecular Dimensions, Newmarket, UK), diffraction quality crystals were obtained from 0.2 M magnesium chloride, 0.1 M Tris-HCl, pH 8.5, supplemented with 30% wt/vol PEG 4000 (polyethylene glycol; precipitant solution number 33).
Collection of X-ray diffraction data and data processing
Crystals were mounted in nylon loops (Hampton Research, Aliso Viejo, CA, USA), cryoprotected in Paratone-N (Hampton Research) and flash-cooled by immersion in liquid nitrogen. X-ray diffraction data were collected at - 173°C using synchrotron radiation on the beamline X06DA (Swiss Light Source, Paul Scherrer Institut, Villigen, Switzerland) with a 1.0-Å monochromatic fixed wavelength and a mar225 charge-coupled device X-ray detector (Marresearch/Rayonix, Norderstedt, Germany). A set of 190 images was recorded with a 0.5° oscillation angle, an exposure time of 2.5 seconds per image and a crystal-to-detector distance of 320 mm. Data were indexed, integrated and scaled with XDS (30 March 2013 version). The space group was determined using POINTLESS. Unit cell content analysis and data reduction were performed with tools from the CCP4 suite v6.02. Data collection and processing statistics are reported in Additional file1.
Structure solution and refinement
Phases were obtained by molecular replacement with the structure of the 25-D1.16 Fab as a model (Protein Data Bank (PDB) ID 3CVI) using Phaser software. Verification of correct packing of the obtained solutions as well as preparation of figures of solved structure was performed using PyMOL (PyMOL Molecular Graphics System, Version 184.108.40.206; Schrödinger, New York, NY, USA). The initial model obtained by molecular replacement was further refined against X-ray data by successive runs of the REFMAC5 program, followed by the manual model adjustments in Coot software. The jelly body refinement option of REFMAC5 with noncrystallographic symmetry (NCS) constraints was used. Following model completion, the NCS constraints were removed. Water molecules were added manually into a positive difference electron density in the Coot environment. Because of the missing electron density, the side chains of complementarity determining region (CDR) L1 and of a surface loop in the heavy-chain constant domain were only partially modelled. The progress of refinement was monitored by the drop in the values of R-Work and R-Free parameters and root-mean-square deviation of structure characteristics. The final model was verified using the MolProbity server and was of better quality than 98% of structures with similar resolution. The final model and structure factors were deposited in the PDB under ID 4OZ4. The model contains two independently refined DC8E8 Fab molecules and eight water molecules, which were individually checked for a reasonable electron density and correct hydrogen bonding. Refinement statistics are reported in Additional file2.
Affinity and kinetics determination by surface plasmon resonance
A Biacore 3000 instrument with a Sensor Chip CM5 (GE Healthcare Bio-Sciences, Uppsala, Sweden) was used. Amine-coupling reagents (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, N-hydroxysuccinimide, ethanolamine, pH 8.5), P20 detergent and 10 mM sodium acetate pH 5.0 were obtained from GE Healthcare Bio-Sciences. All experiments were performed at 25°C in PBS (pH 7.4) with 0.005% of P20 (PBS-P) as the running buffer. Typically, a quantity of 5,000 response units (RU) of polyclonal anti-mouse antibody (Z0420; DakoCytomation, Glostrup, Denmark) was coupled at pH 5.0 via primary amines simultaneously in two flow cells, one of which was used as a reference in measurement. In each analysis cycle, DC8E8 was captured in the analysed flow cell to reach an immobilisation level of 230 to 250 RU. For the Kd determination, as well as for the determination of kinetic rate constants, twofold serial dilutions of tau proteins, including PBS-P as a control, were injected at a flow rate 50 μl/min over the Sensor Chip. Kinetic binding data were double-referenced and fitted (using BIAevaluation software version 4.1; GE Healthcare Bio-Sciences) to a two-phase reaction model. Kinetic rate constants were approximated globally, maximal responses were fitted locally and bulk response was set to zero.
Transgenic mice were generated to overexpress mis-disordered tau protein 151-391/3R under the control of the mouse Thy1 promoter. Transgenic animals were born and bred in our animal facility and housed in standard laboratory conditions in plastic cages in a temperature- and humidity-controlled environment with a 12:12-hour light-dark cycle and food and water available ad libitum. Efforts were made to minimise the number of animals utilised.
Vaccine was administered once per week when the mice were between 2 and 6 months of age. Purified antibody DC8E8 in PBS was injected intraperitoneally (1 mg of antibody/300-μl dose/animal) into the transgenic mice of the strain R3m/4 expressing human truncated tau. As a control, irrelevant antibody DC51 was injected into R3m/4 transgenic mice following the same vaccination regime and dosing.
Isolation of soluble tau and sarkosyl-insoluble tau
Brain tissue was homogenized in tenfold weight excess of ice-cold extraction buffer (20 mM Tris, pH 7.4, 800 mM NaCl, 1 mM ethylene glycol tetraacetic acid), 1 mM ethylenediaminetetraacetic acid 0.5% β-mercaptoethanol, 10% sucrose, 1 mM Na3VO4 and 20 mM NaF, supplemented with a cOmplete Protease Inhibitor Cocktail Tablet (Roche Diagnostics). After incubation on ice for 5 minutes, the homogenates were cleared by centrifugation at 20,000 g for 20 minutes at 4°C. The supernatants were collected, and the total protein concentration was determined using a Bio-Rad protein assay (Bio-Rad Laboratories, Hercules, CA, USA). This supernatant (designed 1S) contained soluble tau fraction. Subsequently, solid sarkosyl (N-lauroylsarcosine sodium salt; Sigma-Aldrich) was added to the 1S supernatant to achieve 1% concentration and then stirred for 1 hour. Thereafter it was centrifuged at 100,000 g for 1.5 hours at room temperature (RT). Following centrifugation, pellets were gently rinsed with 1 ml of the extraction buffer and centrifuged at 100,000 g for 20 minutes at RT. The pellets containing sarkosyl-insoluble tau fractions were dissolved in SDS-PAGE loading buffer to a final volume corresponding to the 1/50 volume of the 1S supernatant.
Immunoblot analysis of soluble tau and sarcosyl-insoluble tau
Samples of sarkosyl-insoluble tau fractions were dissolved in 1× SDS sample loading buffer in a 1/50 volume of the soluble fraction and heated at 95°C for 5 minutes. Each sample (6 μl) was then loaded onto 5–20% gradient SDS polyacrylamide gels and electrophoresed in a Tris-glycine-SDS buffer system for 40 minutes at 25 mA. Proteins were transferred to PVDF membrane (1 hour at 150 mA in 10 mM N-cyclohexyl-3-aminopropanesulfonic acid, pH 12), and, after blocking in 5% fat-free dry milk in PBS for 1 hour at room temperature, the membrane was incubated for 1 hour with pan-tau mAb DC25. After washes, HRP-conjugated goat anti-mouse Ig (Dako Denmark) diluted 1:4,000 in PBS was used as a secondary antibody. Incubation (1 hour at room temperature) was followed by washing with 0.2% IGEPAL (Sigma-Aldrich) in PBS. The blots were developed with SuperSignal West Pico Chemiluminescent Substrate (Pierce Biotechnology), and the signal was detected using the LAS3000 imaging system. The signal intensities were quantified using AIDA software and then statistically evaluated using an unpaired t-test.
Immunohistochemistry of mouse brain tissue samples
Transgenic mice were deeply anaesthetised with Zoletil-xylazine and perfused intracardially using a peristaltic pump for 2 minutes with PBS. The brain was postfixed overnight in 4% paraformaldehyde in PBS, pH 7.2, cryoprotected with 15% and 25% sucrose solutions (subsequently overnight), frozen in 2-methylbutane (30 seconds at −42°C) and transferred to dry ice. Sagittal brainstem sections (40 μm thick) were cut on a Leica CM1850 cryomicrotome (Leica Biosystems, Buffalo Grove, IL, USA). For semiquantitative analysis, two sections were quantified. Tissue sections were incubated with primary antibodies AT8 (Pierce Endogen) and pS214 (Invitrogen, Carlsbad, CA, USA) overnight at 4°C. Sections were immunostained using the standard avidin-biotin-peroxidase method (VECTASTAIN Elite ABC Kit; Vector Laboratories, Burlingame, CA, USA) with VIP as the chromogen.
Immunohistochemistry of human brain tissue samples
Human brain tissue samples were obtained from Netherlands Brain Bank in accordance with local ethical approval and written consent from the donors or their next of kin. Ethical approval was obtained for the analyses carried out using this tissue (Institute of Neuroimmunology, Slovak Academy of Sciences, 5/2011). Immunohistochemical staining was performed on paraffin sections after deparaffinisation. The brain sections were treated with 99% formic acid for 1 minute and then in a pressure cooker for 20 minutes. Afterwards, sections were incubated for 20 minutes at room temperature in 1% H2O2, followed by a 30-minute incubation in blocking solution (0.01 M PBS containing 0.3% Triton X-100 and 1% horse serum). This was followed by overnight incubation at 4°C in primary antibody DC8E8 in blocking solution. After washing, the sections were incubated in biotinylated secondary antibody (VECTASTAIN; Vector Laboratories). The reaction product was visualised using avidin-biotin and Vector VIP as the chromogen (Vector Laboratories).
Statistical analysis was carried out using the Prism statistical software package (GraphPad Software, La Jolla, CA, USA). To compare two groups, the Mann–Whitney U test or an unpaired t-test was applied. The results are presented as mean ± standard error of the mean unless otherwise specified. Differences were considered significant at the level of P < 0.05.
DC8E8 candidate therapeutic antibody is inhibiting pathological tau–tau interactions
Screening of monoclonal antibodies that inhibit pathological tau–tau interaction revealed DC8E8 as the best candidate a
Inhibition of tau oligomer formation (%)
DC8E8 recognises neurofibrillary pathology in preclinical, clinically incipient and fully developed Alzheimer’s disease
DC8E8 immunotherapy significantly reduces levels of insoluble tau oligomers in transgenic mouse brain
DC8E8 immunotherapy decreases load of neurofibrillary tangles in brains of transgenic mice expressing mis-disordered tau
DC8E8 targets four separate structural determinants essential for pathological tau–tau interaction
To define the minimal epitopes recognised by DC8E8, additional tau peptides (5-mers, 6-mers, 7-mers and 12-mers) derived from each MTBR were designed. All peptides were analysed for their ability to compete with mis-disordered tau (151-391/4R) for binding to DC8E8. As shown in Figure 5B, 6-mer peptides derived from four MTBRs (tau 268–273 from MTBR1, tau 299–304 from MTBR2 identical to tau 362–367 from MTBR4 and tau 330–335 from MTBR3) were able to compete with mis-disordered tau (151-391/4R) for binding to DC8E8. However, removal of either N-terminal histidine (5-mer peptides; tau 269–273, tau 300–304) or the C-terminal glycine (5-mer peptides, tau 268–272, tau 299–303) abolished competition of these peptides with tau (151-391/4R) for binding to DC8E8 (Figure 5B). This shows that the 6-mer peptides with consensus sequence HXPGGG localised in the MTBR region of tau are sufficient for DC8E8 recognition. At the same time, peptides six amino acid residues long encompassing the consensus sequence HXPGGG are necessary to create the three dimensional structure recognized by DC8E8. Thus, we can draw three conclusions. (1) The HXPGGG amino acid sequence represents the minimal epitope, structural determinant on mis-disordered protein tau recognised by DC8E8. (2) The minimal DC8E8 epitope on mis-disordered human tau is present four times as four separate structural determinants, epitopes, each encompassing six amino acids. (3) The primary structure of epitope 1 is 268HQPGGG273 (located within MTBR1), that of epitope 2 is 299HVPGGG304 (located within MTBR2), that of epitope 3 is 330HKPGGG335 (located within MTBR3) and that of epitope 4 is 362HVPGGG367 (located within MTBR4) (Figure 5C).
Atomic structure of DC8E8 binding site recognizing determinants of pathological tau–tau interaction
DC8E8 kinetically prefers interaction with truncated tau proteins
AD is the leading cause of senile dementia. Its prevalence is predicted to increase at least threefold between 2000 and 2050, rendering AD an increasing worldwide public health problem. The current most effective treatment approach for AD—cholinesterase inhibitors and N-methyl-D-aspartate receptor antagonists—is purely symptomatic and provides benefit for only up to 12 months. Therefore, the aim of the currently proposed therapeutic approaches and strategies for AD is to counteract the fundamental underlying pathological processes leading to the development and progression of the disease.
The main neuropathological hallmarks of AD are NFTs composed of tau protein and senile plaques consisting of amyloid-β. It has been shown that diseased modified tau proteins play an essential role in the clinical manifestation of AD[7, 9, 56]. An increased understanding of the molecular mechanisms underlying the pathological transformations of tau has opened up the possibility of specifically targeting pathologically modified tau protein for therapeutic purposes. As a result, a number of therapeutic approaches that directly or indirectly target the tau misfolding cascade have emerged[2, 57, 58]. One of these promising therapeutic approaches is immunotherapy targeting various tau species. Several independent studies have shown that either active or passive immunotherapy could prevent tau aggregation or clear tau aggregates and reduce tau hyperphosphorylation[22–24, 26, 59–62]. However, it has been discussed that phosphorylated tau antigens, used predominantly in the abovementioned studies, display some potential risks, as these phosphorylation sites are associated mainly with matured NFTs and not with early stages of tangle formation. Moreover, phosphorylation is the main physiological mechanism regulating tau structure and function, and, therefore, the major concern caused by active immunisation with phospho-tau peptides is an immune response towards the physiological tau species.
In this report, we present a preclinical immunoproteomic platform for the identification of structural determinants on tau protein essential for its pathological interaction. Our strategy was based on our findings that truncation of tau protein significantly changes the structure and function of the molecule. Thus, mis-disordered truncated tau is a substrate for pathological tau–tau interactions[27, 30, 31]. To identify the critical structural determinants on tau responsible for pathological tau–tau interaction, we developed molecular imprinting technology based on the unique properties of mAbs recognizing structural changes of naturally disordered proteins. Using hypothesis-driven research approaches, we proposed a targeted vaccine development strategy consisting of the following steps: (1) identification of a mAb that is able to prevent tau from its pathological tau–tau interaction, (2) in vivo validation of the therapeutic activity of the antibody with special emphasis on reduction of neurofibrillary pathology and sarcosyl-insoluble tau, (3) identification of the epitopes on tau recognised by therapeutic antibody, (4) identification of the three-dimensional structure of these binding sites and (5) characterisation of the antibody’s ability to discriminate between disease-modified and physiological tau protein. The chosen algorithm allows us to identify structural determinants essential for pathological tau–tau interaction and validate it as a novel therapeutic target for tau immunotherapy in AD.
In order to identify an antibody that can protect tau from pathological tau assembly, we have developed an in vitro assay for screening of antibody potency to inhibit pathological tau–tau interaction. Previously, it has been shown that the key regions responsible for tau–tau interactions are located in the microtubule-binding domain of tau[37, 64, 65]; however, their pathologic gain of function is regulated by the truncation of distant parts of the tau protein molecule. The phenomenon of regulation of protein function by truncation is particularly prominent in the intrinsically disordered proteins involved in neurodegenerative diseases. It has been shown that tau truncation is one of the key pathognomonic features of neurodegenerative processes in AD. Furthermore, removal of tau protein termini triggers a tau misfolding cascade and the development of tau neurofibrillary pathology in animal models[30, 31]. In our present study, we found truncated tau protein 151-391/4R to be particularly suitable for screening for antibodies inhibiting pathological tau–tau interaction. It allowed us to identify DC8E8 antibody as extremely efficient in inhibiting tau–tau interaction. Proteomic mapping revealed that the antibody was capable of binding to four independent and yet highly homologous binding regions, each of which is a separate epitope, one in each of the MTBR regions. DC8E8 represents the first specific antibody recognizing four previously unidentified functional regions of tau (structural determinants, epitopes) regulating pathological tau–tau interaction.
In line with this, DC8E8 inhibits the formation of dimers, trimers and oligomers by mis-disordered tau. Therefore, identified DC8E8 epitopes are involved in tau conformational changes leading to tau oligomerisation as the first step of tau fibrillisation. As DC8E8 is capable of discriminating between pathological and physiological tau proteins, at least one of these four epitopes is conformational.
It is probable that binding of DC8E8 to physiological tau at one or more of these epitopes impedes in the same manner certain conformational changes in the tau that are needed for the oligomerisation of tau. In other words, targeting of these epitopes by antibody influences the structure of adjacent regions with β-structural propensities (for example, 274–281, 306–311). Thus, binding of DC8E8 to one of these four epitopes within normal tau is capable of preventing one of the earliest pathological changes in tau, connected with the formation of β-sheets within tau. All of the four epitopes targeted by DC8E8 are present in the sequence of the AD paired helical filament (PHF) core. Inhibition of these motifs by DC8E8 and subsequent prevention of PHF core formation is a good explanation for the mechanism underlying how DC8E8 is capable of interfering with the multiple tau-mediated activities contributing to AD pathology, including (1) transition from physiological tau to pathological tau; (2) formation of tau dimers, trimers and other tau oligomers; and (3) formation of insoluble tau aggregates.
We have determined the X-ray structure of a DC8E8 binding site revealing a relatively flexible, deep binding pocket. The pronounced flexibility of CDR L1 and CDR H3 (Figure 6A and B) can facilitate recognition of four independent homologous epitopes on tau. The topology of the DC8E8 binding pocket indicates a protruding shape adopted by the HXPGGG epitope in the tau–DC8E8 complex, suggesting a 180° turn on the tau chain. As we have shown that DC8E8 targets the regulatory motif of tau–tau interaction, it is likely that formation of such a protruding turn by the HXPGGG tau sequence and binding of this turn by DC8E8 efficiently blocks interaction of downstream aggregation-prone tau motifs[37, 64, 65].
We have proven the antibody’s therapeutic efficacy in an in vivo model. To date, tau-targeted immunisation has been explored exclusively in different mutant tau mouse models because these mice develop NFTs in their brains. However, no tau mutations have thus far been observed in AD. Tau protein mutations on chromosome 17 correlate with cognitive and motor impairment in frontotemporal dementia linked to chromosome 17[67, 68]. It is important to note, however, that posttranslational modifications of tau protein, such as truncation[37, 69, 70], abnormal hyperphosphorylation, glycosylation, glycation[73, 74], ubiquitination, polyamination and nitration, play key roles in tangle development. They can significantly influence conformational characteristics of tau and impose novel toxic properties onto mis-disordered tau. Thus, in order to test the biological function of the inhibitory antibody DC8E8, we used an animal model of AD based on an AD-relevant disease modification of tau—truncation. Our transgenic mice of the strain R3m/4 express truncated tau derived from human AD brains and display early onset of AD tau pathology, which renders this model an ideal test system for immunotherapy targeting tau neurofibrillary lesions. We treated transgenic animals with DC8E8 for 4 months starting at their second month of life. The antibody was able to significantly reduce early and late tau pathology, showing that DC8E8 targets and disables disease-modified mis-disordered tau. Importantly, antibody DC8E8 recognises all forms of tau lesions, including pretangles and intracellular and extracellular NFTs, in both preclinical and fully developed human AD. Therefore, it is reasonable to expect that the antibody will exert the same pattern of therapeutic activity in AD patients.
We have identified a novel therapeutic target on tau protein that can be utilised for the treatment of AD. On the basis of our study findings, we can draw the following conclusions. (1) The HXPGGG amino acid sequence represents minimal epitope, structural determinant on mis-disordered protein tau recognised by DC8E8. (2) The minimal DC8E8 epitope on mis-disordered human tau is present four times as four separate structural determinants, epitopes, each encompassing six amino acids. (3) The primary structure of epitope 1 is 268HQPGGG273 (located within MTBR1), that of epitope 2 is 299HVPGGG304 (located within MTBR2), that of epitope 3 is 330HKPGGG335 (located within MTBR3) and that of epitope 4 is 362HVPGGG367 (located within MTBR4) (Figure 5C). (4) DC8E8 recognises mis-disordered tau with far higher affinity than physiological tau. Mis-disordered tau displays a DC8E8 high-affinity epitope, and physiological tau displays a DC8E8 low-affinity epitope. (5) Binding of DC8E8 to a high-affinity epitope inhibits pathological tau–tau interaction and thus delineates a key regulatory domain for pathological tau assembly. Binding of DC8E8 to a low-affinity epitope on physiological tau has no impact on its physiological function (promotion of microtubule assembly). The discriminatory potential of DC8E8 in targeting of diseased and healthy tau underlines its use as a safe immunotherapeutic agent. (6) DC8E8 high-affinity epitope represents the most sensitive and vulnerable target for AD immunotherapy identified to date. (7) Because formation of DC8E8 high-affinity epitope is an essential event for pathological transition of tau and is present on every diseased tau molecule, we suggest naming this druggable structure the ‘Achilles heel of diseased tau’.
Complementarity determining region
Enzyme-linked immunosorbent assay
Horse radish peroxidase
Protein Data Bank
Surface plasmon resonance.
For access to Swiss Light Source (SLS), we acknowledge the support of the European Community Research Infrastructure Action under FP7, grant agreement 226716.
- Kosik KS, Shimura H: Phosphorylated tau and the neurodegenerative foldopathies. Biochim Biophys Acta. 2005, 1739: 298-310.View ArticlePubMedGoogle Scholar
- Zilka N, Kontsekova E, Novak M: Chaperone-like antibodies targeting misfolded tau protein: new vistas in the immunotherapy of neurodegenerative foldopathies. J Alzheimers Dis. 2008, 15: 169-179.PubMedGoogle Scholar
- Prince M, Bryce R, Albanese E, Wimo A, Ribeiro W, Ferri CP: The global prevalence of dementia: a systematic review and metaanalysis. Alzheimers Dement. 2013, 9: 63–75.e62-View ArticleGoogle Scholar
- Mangialasche F, Solomon A, Winblad B, Mecocci P, Kivipelto M: Alzheimer’s disease: clinical trials and drug development. Lancet Neurol. 2010, 9: 702-716.View ArticlePubMedGoogle Scholar
- Zilka N, Korenova M, Novak M: Misfolded tau protein and disease modifying pathways in transgenic rodent models of human tauopathies. Acta Neuropathol. 2009, 118: 71-86.View ArticlePubMedGoogle Scholar
- Corbett A, Pickett J, Burns A, Corcoran J, Dunnett SB, Edison P, Hagan JJ, Holmes C, Jones E, Katona C, Kearns I, Kehoe P, Mudher A, Passmore A, Shepherd N, Walsh F, Ballard C: Drug repositioning for Alzheimer’s disease. Nat Rev Drug Discov. 2012, 11: 833-846.View ArticlePubMedGoogle Scholar
- Braak H, Braak E: Neuropathological stageing of Alzheimer-related changes. Acta Neuropathol. 1991, 82: 239-259.View ArticlePubMedGoogle Scholar
- Duyckaerts C: Disentangling Alzheimer’s disease. Lancet Neurol. 2011, 10: 774-775.View ArticlePubMedGoogle Scholar
- Nelson PT, Alafuzoff I, Bigio EH, Bouras C, Braak H, Cairns NJ, Castellani RJ, Crain BJ, Davies P, Del Tredici K, Duyckaerts C, Frosch MP, Haroutunian V, Hof PR, Hulette CM, Hyman BT, Iwatsubo T, Jellinger KA, Jicha GA, Kövari E, Kukull WA, Leverenz JB, Love S, Mackenzie IR, Mann DM, Masliah E, McKee AC, Montine TJ, Morris JC, Schneider JA: Correlation of Alzheimer disease neuropathologic changes with cognitive status: a review of the literature. J Neuropathol Exp Neurol. 2012, 71: 362-381.PubMed CentralView ArticlePubMedGoogle Scholar
- Murray ME, Graff-Radford NR, Ross OA, Petersen RC, Duara R, Dickson DW: Neuropathologically defined subtypes of Alzheimer’s disease with distinct clinical characteristics: a retrospective study. Lancet Neurol. 2011, 10: 785-796.PubMed CentralView ArticlePubMedGoogle Scholar
- Braak H, Del Tredici K: The pathological process underlying Alzheimer’s disease in individuals under thirty. Acta Neuropathol. 2011, 121: 171-181.View ArticlePubMedGoogle Scholar
- Duyckaerts C: Tau pathology in children and young adults: Can you still be unconditionally baptist?. Acta Neuropathol. 2011, 121: 145-147.View ArticlePubMedGoogle Scholar
- Schönheit B, Zarski R, Ohm TG: Spatial and temporal relationships between plaques and tangles in Alzheimer-pathology. Neurobiol Aging. 2004, 25: 697-711.View ArticlePubMedGoogle Scholar
- Whitwell JL, Josephs KA, Murray ME, Kantarci K, Przybelski SA, Weigand SD, Vemuri P, Senjem ML, Parisi JE, Knopman DS, Boeve BF, Petersen RC, Dickson DW, Jack CR: MRI correlates of neurofibrillary tangle pathology at autopsy: a voxel-based morphometry study. Neurology. 2008, 71: 743-749.PubMed CentralView ArticlePubMedGoogle Scholar
- Bancher C, Jellinger KA: Neurofibrillary tangle predominant form of senile dementia of Alzheimer type: a rare subtype in very old subjects. Acta Neuropathol. 1994, 88: 565-570.View ArticlePubMedGoogle Scholar
- Cairns NJ, Bigio EH, Mackenzie IR, Neumann M, Lee VM, Hatanpaa KJ, White CL, Schneider JA, Grinberg LT, Halliday G, Duyckaerts C, Lowe JS, Holm IE, Tolnay M, Okamoto K, Yokoo H, Murayama S, Woulfe J, Munoz DG, Dickson DW, Ince PG, Trojanowski JQ, Mann DM, Consortium for Frontotemporal Lobar Degeneration: Neuropathologic diagnostic and nosologic criteria for frontotemporal lobar degeneration: consensus of the Consortium for Frontotemporal Lobar Degeneration. Acta Neuropathol. 2007, 114: 5-22.PubMed CentralView ArticlePubMedGoogle Scholar
- Josephs KA, Petersen RC, Knopman DS, Boeve BF, Whitwell JL, Duffy JR, Parisi JE, Dickson DW: Clinicopathologic analysis of frontotemporal and corticobasal degenerations and PSP. Neurology. 2006, 66: 41-48.View ArticlePubMedGoogle Scholar
- Kouri N, Murray ME, Hassan A, Rademakers R, Uitti RJ, Boeve BF, Graff-Radford NR, Wszolek ZK, Litvan I, Josephs KA, Dickson DW: Neuropathological features of corticobasal degeneration presenting as corticobasal syndrome or Richardson syndrome. Brain. 2011, 134: 3264-3275.PubMed CentralView ArticlePubMedGoogle Scholar
- Tolnay M, Sergeant N, Ghestem A, Chalbot S, De Vos RA, Jansen Steur EN, Probst A, Delacourte A: Argyrophilic grain disease and Alzheimer’s disease are distinguished by their different distribution of tau protein isoforms. Acta Neuropathol. 2002, 104: 425-434.PubMedGoogle Scholar
- Williams DR, Lees AJ: Progressive supranuclear palsy: clinicopathological concepts and diagnostic challenges. Lancet Neurol. 2009, 8: 270-279.View ArticlePubMedGoogle Scholar
- Yamada M: Senile dementia of the neurofibrillary tangle type (tangle-only dementia): neuropathological criteria and clinical guidelines for diagnosis. Neuropathology. 2003, 23: 311-317.View ArticlePubMedGoogle Scholar
- Asuni AA, Boutajangout A, Quartermain D, Sigurdsson EM: Immunotherapy targeting pathological tau conformers in a tangle mouse model reduces brain pathology with associated functional improvements. J Neurosci. 2007, 27: 9115-9129.View ArticlePubMedGoogle Scholar
- Bi M, Ittner A, Ke YD, Götz J, Ittner LM: Tau-targeted immunization impedes progression of neurofibrillary histopathology in aged P301L tau transgenic mice. PLoS One. 2011, 6: e26860-PubMed CentralView ArticlePubMedGoogle Scholar
- Boutajangout A, Quartermain D, Sigurdsson EM: Immunotherapy targeting pathological tau prevents cognitive decline in a new tangle mouse model. J Neurosci. 2010, 30: 16559-16566.PubMed CentralView ArticlePubMedGoogle Scholar
- Richter M, Hoffmann R, Singer D: T-cell epitope-dependent immune response in inbred (C57BL/6 J, SJL/J, and C3H/HeN) and transgenic P301S and Tg2576 mice. J Pept Sci. 2013, 19: 441-451.View ArticlePubMedGoogle Scholar
- Troquier L, Caillierez R, Burnouf S, Fernandez-Gomez FJ, Grosjean ME, Zommer N, Sergeant N, Schraen-Maschke S, Blum D, Buee L: Targeting phospho-Ser422 by active Tau immunotherapy in the THYTau22 mouse model: a suitable therapeutic approach. Curr Alzheimer Res. 2012, 9: 397-405.PubMed CentralView ArticlePubMedGoogle Scholar
- Kovacech B, Skrabana R, Novak M: Transition of tau protein from disordered to misordered in Alzheimer’s disease. Neurodegener Dis. 2010, 7: 24-27.View ArticlePubMedGoogle Scholar
- Kontseková E, Novák M, Kontsek P, Borecký L, Lesso J: The effect of postfusion cell density on establishment of hybridomas. Folia Biol (Praha). 1988, 34: 18-22.Google Scholar
- Vechterova L, Kontsekova E, Zilka N, Ferencik M, Ravid R, Novak M: DC11: a novel monoclonal antibody revealing Alzheimer’s disease-specific tau epitope. Neuroreport. 2003, 14: 87-91.View ArticlePubMedGoogle Scholar
- Filipcik P, Zilka N, Bugos O, Kucerak J, Koson P, Novak P, Novak M: First transgenic rat model developing progressive cortical neurofibrillary tangles. Neurobiol Aging. 2012, 33: 1448-1456.View ArticlePubMedGoogle Scholar
- Zilka N, Filipcik P, Koson P, Fialova L, Skrabana R, Zilkova M, Rolkova G, Kontsekova E, Novak M: Truncated tau from sporadic Alzheimer’s disease suffices to drive neurofibrillary degeneration in vivo. FEBS Lett. 2006, 580: 3582-3588.View ArticlePubMedGoogle Scholar
- Zilka N, Kovacech B, Barath P, Kontsekova E, Novák M: The self-perpetuating tau truncation circle. Biochem Soc Trans. 2012, 40: 681-686.View ArticlePubMedGoogle Scholar
- Nakamura K, Greenwood A, Binder L, Bigio EH, Denial S, Nicholson L, Zhou XZ, Lu KP: Proline isomer-specific antibodies reveal the early pathogenic tau conformation in Alzheimer’s disease. Cell. 2012, 149: 232-244.PubMed CentralView ArticlePubMedGoogle Scholar
- Hanes J, Zilka N, Bartkova M, Caletkova M, Dobrota D, Novak M: Rat tau proteome consists of six tau isoforms: implication for animal models of human tauopathies. J Neurochem. 2009, 108: 1167-1176.View ArticlePubMedGoogle Scholar
- Koson P, Zilka N, Kovac A, Kovacech B, Korenova M, Filipcik P, Novak M: Truncated tau expression levels determine life span of a rat model of tauopathy without causing neuronal loss or correlating with terminal neurofibrillary tangle load. Eur J Neurosci. 2008, 28: 239-246.View ArticlePubMedGoogle Scholar
- Zilkova M, Zilka N, Kovac A, Kovacech B, Skrabana R, Skrabanova M, Novak M: Hyperphosphorylated truncated protein tau induces caspase-3 independent apoptosis-like pathway in the Alzheimer’s disease cellular model. J Alzheimers Dis. 2011, 23: 161-169.PubMedGoogle Scholar
- Novak M, Kabat J, Wischik CM: Molecular characterization of the minimal protease resistant tau unit of the Alzheimer’s disease paired helical filament. EMBO J. 1993, 12: 365-370.PubMed CentralPubMedGoogle Scholar
- Skrabana R, Kontsek P, Mederlyova A, Iqbal K, Novak M: Folding of Alzheimer’s core PHF subunit revealed by monoclonal antibody 423. FEBS Lett. 2004, 568: 178-182.View ArticlePubMedGoogle Scholar
- Máciková I, Dedek L, Vrzal V, Kontseková E, Kontsek P, Ciampor F, Novák M: Common and different antigenic properties of the rabies virus glycoprotein of strains SAD-Vnukovo and Pitman-Moore. Acta Virol. 1992, 36: 541-550.PubMedGoogle Scholar
- Cehlar O, Skrabana R, Kovac A, Kovacech B, Novak M: Crystallization and preliminary X-ray diffraction analysis of tau protein microtubule-binding motifs in complex with Tau5 and DC25 antibody Fab fragments. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012, F68: 1181-1185.View ArticleGoogle Scholar
- Kabsch W: XDS. Acta Crystallogr D Biol Crystallogr. 2010, D66: 125-132.View ArticleGoogle Scholar
- Evans PR: An introduction to data reduction: space-group determination, scaling and intensity statistics. Acta Crystallogr D Biol Crystallogr. 2011, D67: 282-292.View ArticleGoogle Scholar
- Winn MD, Ballard CC, Cowtan KD, Dodson EJ, Emsley P, Evans PR, Keegan RM, Krissinel EB, Leslie AG, McCoy A, McNicholas SJ, Murshudov GN, Pannu NS, Potterton EA, Powell HR, Read RJ, Vagin A, Wilson KS: Overview of the CCP4 suite and current developments. Acta Crystallogr D Biol Crystallogr. 2011, D67: 235-242.View ArticleGoogle Scholar
- Mareeva T, Martinez-Hackert E, Sykulev Y: How a T cell receptor-like antibody recognizes major histocompatibility complex-bound peptide. J Biol Chem. 2008, 283: 29053-29059.PubMed CentralView ArticlePubMedGoogle Scholar
- McCoy AJ, Grosse-Kunstleve RW, Adams PD, Winn MD, Storoni LC, Read RJ: Phaser crystallographic software. J Appl Crystallogr. 2007, 40: 658-674.PubMed CentralView ArticlePubMedGoogle Scholar
- Murshudov GN, Skubák P, Lebedev AA, Pannu NS, Steiner RA, Nicholls RA, Winn MD, Long F, Vagin AA: REFMAC 5 for the refinement of macromolecular crystal structures. Acta Crystallogr D Biol Crystallogr. 2011, D67: 355-367.View ArticleGoogle Scholar
- Emsley P, Lohkamp B, Scott WG, Cowtan K: Features and development of Coot. Acta Crystallogr D Biol Crystallogr. 2010, D66: 486-501.View ArticleGoogle Scholar
- Chen VB, Arendall WB, Headd JJ, Keedy DA, Immormino RM, Kapral GJ, Murray LW, Richardson JS, Richardson DC: MolProbity: all-atom structure validation for macromolecular crystallography. Acta Crystallogr D Biol Crystallogr. 2010, D66: 12-21.View ArticleGoogle Scholar
- Myszka DG: Improving biosensor analysis. J Mol Recognit. 1999, 12: 279-284.View ArticlePubMedGoogle Scholar
- Braak E, Braak H, Mandelkow EM: A sequence of cytoskeleton changes related to the formation of neurofibrillary tangles and neuropil threads. Acta Neuropathol. 1994, 87: 554-567.View ArticlePubMedGoogle Scholar
- Braak H, Thal DR, Ghebremedhin E, Del Tredici K: Stages of the pathologic process in Alzheimer disease: age categories from 1 to 100 years. J Neuropathol Exp Neurol. 2011, 70: 960-969.View ArticlePubMedGoogle Scholar
- Delobel P, Lavenir I, Fraser G, Ingram E, Holzer M, Ghetti B, Spillantini MG, Crowther RA, Goedert M: Analysis of tau phosphorylation and truncation in a mouse model of human tauopathy. Am J Pathol. 2008, 172: 123-131.PubMed CentralView ArticlePubMedGoogle Scholar
- Allen B, Ingram E, Takao M, Smith MJ, Jakes R, Virdee K, Yoshida H, Holzer M, Craxton M, Emson PC, Atzori C, Migheli A, Crowther RA, Ghetti B, Spillantini MG, Goedert M: Abundant tau filaments and nonapoptotic neurodegeneration in transgenic mice expressing human P301S tau protein. J Neurosci. 2002, 22: 9340-9351.PubMedGoogle Scholar
- Jeganathan S, von Bergen M, Brutlach H, Steinhoff HJ, Mandelkow E: Global hairpin folding of tau in solution. Biochemistry. 2006, 45: 2283-2293.View ArticlePubMedGoogle Scholar
- Sloane PD, Zimmerman S, Suchindran C, Reed P, Wang L, Boustani M, Sudha S: The public health impact of Alzheimer’s disease, 2000-2050: potential implication of treatment advances. Annu Rev Public Health. 2002, 23: 213-231.View ArticlePubMedGoogle Scholar
- Dickey CA, Petrucelli L: Current strategies for the treatment of Alzheimer’s disease and other tauopathies. Expert Opin Ther Targets. 2006, 10: 665-676.View ArticlePubMedGoogle Scholar
- Götz J, Ittner A, Ittner LM: Tau-targeted treatment strategies in Alzheimer’s disease. Br J Pharmacol. 2012, 165: 1246-1259.PubMed CentralView ArticlePubMedGoogle Scholar
- Panza F, Frisardi V, Solfrizzi V, Imbimbo BP, Logroscino G, Santamato A, Greco A, Seripa D, Pilotto A: Immunotherapy for Alzheimer’s disease: from anti-β-amyloid to tau-based immunization strategies. Immunotherapy. 2012, 4: 213-238.View ArticlePubMedGoogle Scholar
- Boimel M, Grigoriadis N, Lourbopoulos A, Haber E, Abramsky O, Rosenmann H: Efficacy and safety of immunization with phosphorylated tau against neurofibrillary tangles in mice. Exp Neurol. 2010, 224: 472-485.View ArticlePubMedGoogle Scholar
- Boutajangout A, Ingadottir J, Davies P, Sigurdsson EM: Passive immunization targeting pathological phospho-tau protein in a mouse model reduces functional decline and clears tau aggregates from the brain. J Neurochem. 2011, 118: 658-667.PubMed CentralView ArticlePubMedGoogle Scholar
- Theunis C, Crespo-Biel N, Gafner V, Pihlgren M, López-Deber MP, Reis P, Hickman DT, Adolfsson O, Chuard N, Ndao DM, Borghgraef P, Devijver H, Van Leuven F, Pfeifer A, Muhs A: Efficacy and safety of a liposome-based vaccine against protein Tau, assessed in tau.P301L mice that model tauopathy. PLoS One. 2013, 8: e72301-PubMed CentralView ArticlePubMedGoogle Scholar
- Yanamandra K, Kfoury N, Jiang H, Mahan TE, Ma S, Maloney SE, Wozniak DF, Diamond MI, Holtzman DM: Anti-tau antibodies that block tau aggregate seeding in vitro markedly decrease pathology and improve cognition in vivo. Neuron. 2013, 80: 402-414.PubMed CentralView ArticlePubMedGoogle Scholar
- Kayed R: Anti-tau oligomers passive vaccination for the treatment of Alzheimer disease. Hum Vaccin. 2010, 6: 931-935.View ArticlePubMedGoogle Scholar
- Sawaya MR, Sambashivan S, Nelson R, Ivanova MI, Sievers SA, Apostol MI, Thompson MJ, Balbirnie M, Wiltzius JJ, McFarlane HT, Madsen AØ, Riekel C, Eisenberg D: Atomic structures of amyloid cross-β spines reveal varied steric zippers. Nature. 2007, 447: 453-457.View ArticlePubMedGoogle Scholar
- von Bergen M, Friedhoff P, Biernat J, Heberle J, Mandelkow EM, Mandelkow E: Assembly of tau protein into Alzheimer paired helical filaments depends on a local sequence motif (306VQIVYK311) forming β structure. Proc Natl Acad Sci USA. 2000, 97: 5129-5134.PubMed CentralView ArticlePubMedGoogle Scholar
- Jadhav S, Zilka N, Novak M: Protein truncation as a common denominator of human neurodegenerative foldopathies. Mol Neurobiol. 2013, 48: 516-532.View ArticlePubMedGoogle Scholar
- Poorkaj P, Bird TD, Wijsman E, Nemens E, Garruto RM, Anderson L, Andreadis A, Wiederholt WC, Raskind M, Schellenberg GD: Tau is a candidate gene for chromosome 17 frontotemporal dementia. Ann Neurol. 1998, 43: 815-825.View ArticlePubMedGoogle Scholar
- Spillantini MG, Bird TD, Ghetti B: Frontotemporal dementia and Parkinsonism linked to chromosome 17: a new group of tauopathies. Brain Pathol. 1998, 8: 387-402.View ArticlePubMedGoogle Scholar
- Novak M: Truncated tau protein as a new marker for Alzheimer’s disease. Acta Virol. 1994, 38: 173-189.PubMedGoogle Scholar
- Novak M, Jakes R, Edwards PC, Milstein C, Wischik CM: Difference between the tau protein of Alzheimer paired helical filament core and normal tau revealed by epitope analysis of monoclonal antibodies 423 and 7.51. Proc Natl Acad Sci USA. 1991, 88: 5837-5841.PubMed CentralView ArticlePubMedGoogle Scholar
- Grundke-Iqbal I, Iqbal K, Tung YC, Quinlan M, Wisniewski HM, Binder LI: Abnormal phosphorylation of the microtubule-associated protein τ (tau) in Alzheimer cytoskeletal pathology. Proc Natl Acad Sci USA. 1986, 83: 4913-4917.PubMed CentralView ArticlePubMedGoogle Scholar
- Wang JZ, Grundke-Iqbal I, Iqbal K: Glycosylation of microtubule-associated protein tau: an abnormal posttranslational modification in Alzheimer’s disease. Nat Med. 1996, 2: 871-875.View ArticlePubMedGoogle Scholar
- Ledesma MD, Bonay P, Colaco C, Avila J: Analysis of microtubule-associated protein tau glycation in paired helical filaments. J Biol Chem. 1994, 269: 21614-21619.PubMedGoogle Scholar
- Yan SD, Chen X, Schmidt AM, Brett J, Godman G, Zou YS, Scott CW, Caputo C, Frappier T, Smith MA, Perry G, Yen SH, Stern D: Glycated tau protein in Alzheimer disease: a mechanism for induction of oxidant stress. Proc Natl Acad Sci USA. 1994, 91: 7787-7791.PubMed CentralView ArticlePubMedGoogle Scholar
- Bondareff W, Wischik CM, Novak M, Amos WB, Klug A, Roth M: Molecular analysis of neurofibrillary degeneration in Alzheimer’s disease: an immunohistochemical study. Am J Pathol. 1990, 137: 711-723.PubMed CentralPubMedGoogle Scholar
- Tucholski J, Kuret J, Johnson GV: Tau is modified by tissue transglutaminase in situ: possible functional and metabolic effects of polyamination. J Neurochem. 1999, 73: 1871-1880.PubMedGoogle Scholar
- Horiguchi T, Uryu K, Giasson BI, Ischiropoulos H, LightFoot R, Bellmann C, Richter-Landsberg C, Lee VM, Trojanowski JQ: Nitration of tau protein is linked to neurodegeneration in tauopathies. Am J Pathol. 2003, 163: 1021-1031.PubMed CentralView ArticlePubMedGoogle Scholar
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